The effect of fermented Glycine max (L.) Merr. to rats induced CCl 4 toward hepatic IL-6 expression and ALT-AST level

• Published in: Journal of physics. Conference series 2020-01, Vol.1430 (1), p.12019
• Main Authors: Permata, F S, Roosdiana, A, Anggraini, V O
• Format: Article
• Language: English
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1430/1/012019

Carbon tetrachloride (CCI 4 ) is one of the hepatotoxic compounds that can cause hepatic fibrosis. Fermented soybean ( Glycine max (L.) Merr.) has higher isoflavone and fibrinolytic enzymes that could degrade fibrin substrates. The aim of this research was to know the effect of fermented soybean ( Glycine max (L.) Merr.,) to IL-6 expression and ALT-AST level in rats ( Rattus novergicus ) that induced by CCl 4 . The groups consist of negative control group, positive control group, 3 treatment groups which used dose 200, 400, 800 mg/kg BW. The treatments were conducted 2 mL/day along 14 days. Rats were euthanized then liver and serum were collected. The IL-6 expression of liver was measured by Immunohistochemistry and ASTALT level used spectrophotometer. The parameters were analyzed statistically quantitatively using one-way ANOVA and continued test of Post hoc Tukey (α = 0,05). The result showed that dose of 800 mg/kg BW was an effective dose which has the highest percentage reduction both of hepatic IL-6 expression (68,10%) and ALT-AST level 81,16% and 46,45% respectively. The effect was related isoflavone from fermented soybean that acted as anti-inflammatory. The conclusion was the fermented of soybean able to decrease the hepatic IL-6 expression and ALTAST level in induced CCl 4 rats.