Yeast cells proliferation on various strong static magnetic fields and temperatures

• Published in: Journal of physics. Conference series 2009-03, Vol.156 (1), p.012016
• Main Authors: Otabe, E S, Kuroki, S, Nikawa, J, Matsumoto, Y, Ooba, T, Kiso, K, Hayashi, H
• Format: Article
• Language: English
• Subjects: Coefficients; Counting; Density; Dextrose; Diamagnetism; High temperature; Magnetic fields; Magnetism; Optical microscopes; Peptones; Physics; Shaking; Time dependence; Time measurement; Yeast
• ISSN: 1742-6596; 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/156/1/012016

The effect of strong magnetic fields on activities of yeast cells were investigated. Experimental yeast cells were cultured in 5 ml of YPD(Yeast extract Peptone Dextrose) for the number density of yeast cells of 5.0 ±0.2 x 106/ml with various temperatures and magnetic fields up to 10 T. Since the yeast cells were placed in the center of the superconducting magnet, the effect of magnetic force due to the diamagnetism and magnetic gradient was negligibly small. The yeast suspension was opened to air and cultured in shaking condition. The number of yeast cells in the yeast suspension was counted by a counting plate with an optical microscope, and the time dependence of the number density of yeast cells was measured. The time dependence of the number density of yeast cells, ρ, of initial part is analyzed in terms of Malthus equation as given by ρ ρo exp(kt), where k is the growth coefficient. It is found that, the growth coefficient under the magnetic field is suppressed compared with the control. The growth coefficient decreasing as increasing magnetic field and is saturated at about 5 T. On the other hand, it is found that the suppression of growth of yeast cells by the magnetic field is diminished at high temperatures.