Terpenoid as Antibacterial Produced by Endophyte Fusarium oxysporum LBKURCC41 from Dahlia variabilis Tuber

Fusarium oxysporum LBKURCC41 is an endophytic isolated from Dahlia variabilis tubers from Padang Luar, West Sumatra. The strain was fermented using modified Huangs' media, ie using corn as a carbon source for 15 days. The purpose of this study is to isolate secondary metabolites from LBKURCC41...

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Bibliographic Details
Published in:Journal of physics. Conference series 2020-10, Vol.1655 (1), p.12034
Main Authors: Piska, F, Teruna, H Y, Saryono
Format: Article
Language:English
Subjects:
antibacterial
Antiinfectives and antibacterials
E coli
endophyte
Fermentation
Fusarium oxysporum
Metabolites
NMR spectroscopy
Physics
terpenoid
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Summary:Fusarium oxysporum LBKURCC41 is an endophytic isolated from Dahlia variabilis tubers from Padang Luar, West Sumatra. The strain was fermented using modified Huangs' media, ie using corn as a carbon source for 15 days. The purpose of this study is to isolate secondary metabolites from LBKURCC41 strains. The filtrate from the liquid fermentation media was extracted using EtOAc:MeOH at a ratio of 95:5 to 50:50. Purification using fast chromatography was performed with a mixture of BAW (5:1:4). The structure was elucidated by, FTIR and 1H NMR spectroscopy. The isolate was identified as a sesquiterpenoid, with weak antimicrobial activity against E.coli and S. aureus with 2.1 mm clear zone and concentration of 10 ppm.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/1655/1/012034