Molecular diagnostics using magnetic nanobeads

In this paper, we investigate the volume-amplified magnetic nanobead detection assay with respect to bead size, bead concentration and bead oligonucleotide surface coverage in order to improve the understanding of the underlying microscopic mechanisms. It has been shown that: (i) the immobilization...

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Bibliographic Details
Published in:Journal of physics. Conference series 2010-01, Vol.200 (12), p.122011
Main Authors: Torre, Teresa Zardán Gómez de la, Strömberg, Mattias, Göransson, Jenny, Gunnarsson, Klas, Nilsson, Mats, Svedlindh, Peter, Strømme, Maria
Format: Article
Language:English
Subjects:
Beads
Oligonucleotides
Physics
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Summary:In this paper, we investigate the volume-amplified magnetic nanobead detection assay with respect to bead size, bead concentration and bead oligonucleotide surface coverage in order to improve the understanding of the underlying microscopic mechanisms. It has been shown that: (i) the immobilization efficiency of the beads depends on the surface coverage of oligonucleotides, (ii) by using lower amounts of probe-tagged beads, detection sensitivity can be improved and (iii) using small enough beads enables both turn-off and turn-on detection. Finally, biplex detection was demonstrated.
ISSN:1742-6596
1742-6588
1742-6596
DOI:10.1088/1742-6596/200/12/122011