Chemical characterization of extracellular vesicles of mesenchymal stromal cells: TOF-SIMS and BCARS approach

Paracrine functions of mesenchymal stem (stromal) cells (MSCs) rely, at least partly, on membrane-bound extracellular vesicles (EVs) with rich composition of lipids, nucleic acids and signaling proteins. Elucidation the underlying chemistry could potentially lead to MSCs-free therapy. However, the s...

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Bibliographic Details
Published in:Journal of physics. Conference series 2021-12, Vol.2086 (1), p.12107
Main Authors: Aybush, A V, Gulin, A A, Kuzoiatova, A A, Gubina, M V, Gostev, F E, Syrchina, M S, Ermakov, A S, Suprunenko, E A, Nadtochenko, V A
Format: Article
Language:English
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Summary:Paracrine functions of mesenchymal stem (stromal) cells (MSCs) rely, at least partly, on membrane-bound extracellular vesicles (EVs) with rich composition of lipids, nucleic acids and signaling proteins. Elucidation the underlying chemistry could potentially lead to MSCs-free therapy. However, the secretome of MSCs (EVs’ composition) is non-static and depends on many other factors including surrounding cells and medium. Thus, the research techniques must be able to provide not only bulk but microscopy-scale data within a reasonable time frame. Two of these label-free techniques are subject of this work toward the question of chemical composition of the EVs.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/2086/1/012107