ssDNA damage dependence from singlet oxygen concentration at photodynamic interaction

Single stranded DNA damage at photodynamic treatment with Radachlorin photosensitizer was investigated. Chemical trap method was used to evaluate generation of singlet oxygen in water solution. Interaction of singlet oxygen with ssDNA resulted into decrease of the replication activity of ssDNA. DNA...

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Bibliographic Details
Published in:Journal of physics. Conference series 2017-11, Vol.917 (4), p.42013
Main Authors: Klimenko, V.V., Kaydanov, N.E., Emelyanov, A.K., Bogdanov, A.A.
Format: Article
Language:English
Subjects:
Damage
Physics
Replication
Singlet oxygen
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Summary:Single stranded DNA damage at photodynamic treatment with Radachlorin photosensitizer was investigated. Chemical trap method was used to evaluate generation of singlet oxygen in water solution. Interaction of singlet oxygen with ssDNA resulted into decrease of the replication activity of ssDNA. DNA stopped replicating during PCR at irradiation doses greater than 15 J/cm2 and concentration of photosensitizer [PS] = 3.8 μM. The dependence of replication activity of ssDNA on generated singlet oxygen concentration was identified.
ISSN:1742-6588
1742-6596
1742-6596
DOI:10.1088/1742-6596/917/4/042013