Fabrication of a biocomposite reinforced with hydrophilic eggshell proteins

Soluble eggshell proteins were used as a reinforcing material of electrospun micro/nanofibers for tissue engineering. A biocomposite composed of poly(epsilon-caprolactone) (PCL) micro/nanofibers and soluble eggshell protein was fabricated with a two-step fabrication method, which is an electrospinni...

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Bibliographic Details
Published in:Biomedical materials (Bristol) 2007-12, Vol.2 (4), p.250-256
Main Authors: Kim, GeunHyung, Min, Taijin, Park, Su A, Kim, Wan Doo, Koh, Young Ho
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Cell Culture Techniques - methods
Cells, Cultured
Egg Proteins - chemistry
Egg Proteins - ultrastructure
Electrochemistry - methods
Fibroblasts - cytology
Fibroblasts - physiology
Humans
Hydrophobic and Hydrophilic Interactions
Materials Testing
Nanostructures - chemistry
Nanostructures - ultrastructure
Particle Size
Polyesters - chemistry
Tissue Engineering - methods
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Summary:Soluble eggshell proteins were used as a reinforcing material of electrospun micro/nanofibers for tissue engineering. A biocomposite composed of poly(epsilon-caprolactone) (PCL) micro/nanofibers and soluble eggshell protein was fabricated with a two-step fabrication method, which is an electrospinning process followed by an air-spraying process. To achieve a stable electrospinning process, we used an auxiliary cylindrical electrode connected with a spinning nozzle. PCL biocomposite was characterized in water contact angle and mechanical properties as well as cell proliferation for its application as a tissue engineering material. It showed an improved hydrophilic characteristic compared with that of a micro/nanofiber web generated from a pure PCL solution using a typical electrospinning process. Moreover, the fabricated biocomposite had good mechanical properties compared to a typical electrospun micro/nanofiber mat. The fabricated biocomposite made human dermal fibroblasts grow better than pure PCL. From the results, the reinforced polymeric micro/nanofiber scaffold can be easily achieved with these modified processes.
ISSN:1748-605X
1748-6041
1748-605X
DOI:10.1088/1748-6041/2/4/007