The effect of vitamin e antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen

• Published in: IOP conference series. Earth and environmental science 2022-03, Vol.1001 (1), p.12012
• Main Authors: Bintara, S, Ismaya, I, Widayati, D T, Aji, R N, Asmarawati, W
• Format: Article
• Language: English
• Subjects: Antioxidants; Artificial insemination; Dilution; Goat's milk; Goats; Membranes; Milk; Motility; Peroxidation; Plasma membranes; Reproduction (biology); Semen; Sheep; Sperm; Spermatozoa; Tocopherol; Variance analysis; Viability; Vitamin E; Vitamins
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/1001/1/012012

Artificial insemination supports the prevention of infectious dispersion in organisms which share the same environment. Meanwhile, spermatozoa usually experience a decrease in quality during processing due to the peroxidation process. This study aims to identify the effect of vitamin E antioxidant addition in goat milk diluent on the quality of thin-tailed sheep semen after storing at 5°C for five days. A total of 4 male thin-tailed sheep were used and the treatments consisted of controls, namely goat milk sperm diluent without vitamin E antioxidant (P0), and others with the addition of vitamin E at a dose of 2 gL -1 diluent (P1); 3 gL -1 diluent (P2); and 4 gL -1 (P3). Moreover, the variables observed include individual motility, viability, and intact plasma membrane of spermatozoa after storing at 5°C for five days. The data were analyzed statistically using analysis of variance (ANOVA) with a completely randomized design in one-way. The results showed that the addition of vitamin E antioxidant to goat milk diluent during sperm dilution had a significant effect (P < 0.05) on the motility, viability, and intact plasma membrane. Spermatozoa motility at P0, P1, P2, and P3 were 44.0%, 48.2%, 50.4%, and 53.2%, while the viability was 48.2%, 51.4%, 54.2%, and 56.0%, respectively. Additionally, the intact plasma membranes at P0, P1, P2, and P3 were 41.0%, 46.6%, 48.2%, and 51.4%, respectively. The addition of antioxidant vitamins in goat milk diluent with a concentration of 4 gL -1 produced the best results on sperm quality after cooling. Based on the results, it was concluded that the addition of vitamin E antioxidant to the sperm diluent can improve the quality of thin-tailed sheep chilled semen.