Interaction of plant polysaccharides (inulin, guar gum) with the 20-hydroxyecdysone from quinoa and spinach

• Published in: IOP conference series. Earth and environmental science 2023-08, Vol.1229 (1), p.12035
• Main Authors: Bokov, D O, Bogachuk, M N, Malinkin, A D, Nazarova, V A, Bessonov, V V
• Format: Article
• Language: English
• Subjects: Bioactive compounds; Biodegradation; Biological activity; Ecdysteroids; Guar gum; Gums; Inulin; Leaves; Metabolism; Metabolites; Optimization; Physiological effects; Plant extracts; Polyphenols; Polysaccharides; Quinoa; Raw materials; Saccharides; Secondary metabolites; Seeds; Sorption; Spinach; Sustainability; Vegetables
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/1229/1/012035

The use of plant extracts rich in primary and secondary plant metabolites as biostimulants in agriculture is a modern approach for increasing the sustainability of crops. Quinoa seeds ( Chenopodium quinoa Willd.) and spinach leaves ( Spinacia oleracea L.) are a valuable source of biologically active compounds of plant secondary metabolism such as polyphenolic compounds and ecdysteroids. An important producers of primary plant metabolites – polysaccharides – are common chicory ( Cichorium intybus L.), used to obtain inulin and cluster bean ( Cyamopsis tetragonoloba (L.) Taub.), which accumulates guar gum. The study of the interaction of primary and secondary metabolites of plants is an essential component of research in order to increase their resistance to biodegradation and preserve the optimum of biological action. This paper presents data from a preliminary study of the interactions of components derived from plant raw materials in an in vitro model system. Evaluation of the sorption capacity of plant polysaccharides (inulin, guar gum) was carried out in relation to 20-hydroxyecdysone (20E) – a minor biologically active substance (BAS) of extracts from spinach leaves and quinoa seeds. The sorption ratio of 20E was 92.25-95.87% for the studied concentrations of inulin model solutions. Sorption ratio for a solution of guar gum with a concentration of 0.5% was 94.17-96.00%, in other cases, a complete sorption of 20E was observed. Thus, one should be guided by the optimal ratio of polysaccharides and extracts enriched with 20E to ensure the maximum physiological effect.