Cytological diagnostic of lymphadenitis tuberculosis by eosinophilic material

AFB sputum and chest X-ray are used to identify patients with pulmonary TB. For extrapulmonary TB, fine needle aspiration cytology is needed, even though occasionally found not atypical feature in the form of eosinophilic material with dark brown particles, suspected as TB. This research was to show...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2018-03, Vol.125 (1), p.12049
Main Authors: Delyuzar, Amir, Z, Kusumawati, L
Format: Article
Language:English
Subjects:
Biopsy
Cellular biology
Criteria
Cytology
Deoxyribonucleic acid
Diagnosis
Diagnostic systems
DNA
Leukocytes (eosinophilic)
Lymphadenitis
Polymerase chain reaction
Sensitivity analysis
Sputum
Tuberculosis
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Summary:AFB sputum and chest X-ray are used to identify patients with pulmonary TB. For extrapulmonary TB, fine needle aspiration cytology is needed, even though occasionally found not atypical feature in the form of eosinophilic material with dark brown particles, suspected as TB. This research was to show that eosinophilic material with dark brown particles is accurate as new criteria for the cytological diagnosis of TB. By performing fine needle aspiration biopsy stained with Giemsa, if an eosinophilic material with dark brown particles was encountered, we continued with Ziehl-Neelsen AFB stain and confirmed with PCR. To assess accuracy, we used a diagnostic test to evaluate sensitivity and specificity of eosinophilic material with dark brown particles by using AFB and PCR as the gold standard. The sensitivity and specificity of cytological diagnosis in tuberculosis of eosinophilic material with dark brown particles were 93.65% and 70.99%, respectively if confirmed with AFB. On the other hand, if confirmed with PCR using Mycobacterium tuberculosis DNA, the sensitivity and specificity were 98.95% and 96.79%, respectively. In conclusion, eosinophilic masses with dark brown particles is accurate as new criteria of TB diagnostic cytology with high sensitivity and specificity confirmed with AFB and PCR test.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/125/1/012049