Single Nucleotide Polymorphism of Sex Determining Region-Y Gene Coding Sequences in Belgian Blue Bull and Wagyu Bull Crossbred Cattle

Crossbreeding using exotic bulls have been developed widely in Indonesia. Y-chromosome markers have high value for the evaluation of paternal genetic and phylogeny history. The aim of this study was to investigate the coding sequence polymorphism of SRY gene in crossbred cattle using Belgian Blue Bu...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2020-04, Vol.478 (1), p.12020
Main Authors: Hartatik, T, Bintara, S, Ismaya, I, Panjono, P, Widyobroto, B P, Agus, A, Budisatria, I G S, Leroy, P
Format: Article
Language:English
Subjects:
Amino acids
Cattle
Cross-breeding
Deoxyribonucleic acid
DNA
DNA sequencing
Gene polymorphism
Markers
Mutation
Nucleotide sequence
Nucleotides
Phylogeny
Physical growth
Polymerase chain reaction
Polymorphism
Single-nucleotide polymorphism
Target recognition
Transversion mutation
Y chromosomes
Y gene
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Summary:Crossbreeding using exotic bulls have been developed widely in Indonesia. Y-chromosome markers have high value for the evaluation of paternal genetic and phylogeny history. The aim of this study was to investigate the coding sequence polymorphism of SRY gene in crossbred cattle using Belgian Blue Bull and Wagyu Bull. Polymerase chain reaction method was used to identify the target sequence 690 bp of SRY gene coding sequences. Then, direct DNA sequencing with forward primer was performed to identify the single nucleotide polymorphism (SNP) differences between individual sample. The results of the alignment coding sequence of SRY gene from four GenBank data, Brahman cross cattle, Belgian Blue Bull Cross and Wagyu Bull cross revealed that there are 21 SNPs variation. Six SNPs out of 21 SNPs (29%) was synonymous and 15 SNPs out of 21 SNPs (71%) was change the amino acid (non-synonymous). Eight out of 21 SNPs (38%) were transition mutation and 13 SNPs out of 21 SNPs (62%) were transversion mutation. Crossbred cattle using Brahman cross cow with Belgian Blue Bull and Wagyu Bull show the SNP at position 1707T→G (transversion mutation). This SNP change the amino acid from Phe(TTT) to Cys(TGT). Thus, the result can be used as molecular marker for identifying the paternal lineage of crossbred cattle which recently develop in Indonesia.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/478/1/012020