Comparative analysis of species of the genus Rosa L. on the territory of the Eastern European Plain

The methods of gel electrophoresis with some modification have been developed on the types of rose hips. Identification of a species, as well as determining the degree of its intraspecific polymorphism, includes analysis of individual seeds taken from a random sample of different populations. For id...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-01, Vol.624 (1), p.12015
Main Authors: Ryabinina, Z N, Lyavdanskaya, O A, Bastaeva, G T, Lebedev, S V, Kalyakina, R G, Ryabuhina, M V
Format: Article
Language:English
Subjects:
2-Mercaptoethanol
Comparative analysis
Cultivars
Electrophoresis
Gel electrophoresis
Polyacrylamide
Polymorphism
Polypeptides
Seeds
Sodium
Sodium dodecyl sulfate
Sodium lauryl sulfate
Species
Taxonomy
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Summary:The methods of gel electrophoresis with some modification have been developed on the types of rose hips. Identification of a species, as well as determining the degree of its intraspecific polymorphism, includes analysis of individual seeds taken from a random sample of different populations. For identification and analysis of the purity of the composition of the seeds of cultivars, an analysis of at least 100-150 seeds of each cultivar is recommended. Spectra were recorded using a special digital (large-scale) scale, which allows fixing the position ("addresses") of individual polypeptide components at positions from 1 to 122. Components of low intensity were taken for 1 point, and strong ones (bright) for 2 points. The high sensitivity of the polyacrylamide gel electrophoresis (PAGE) method in the presence of sodium dodecyl sulfate (DS-Na, SDS) and 2-mercaptoethanol to genetic differences at the level of small taxa is clearly manifested in rosehip species. The research results allow us to clarify the taxonomy of the genus Rosa.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/624/1/012015