Anti-Inflammatory Effect and Pharmacological Study of Polygonum Capitatum Based on Network Pharmacology

• Published in: IOP conference series. Earth and environmental science 2021-01, Vol.632 (3), p.32013
• Main Authors: Fang, Rongze, Li, Xiaofen, Kong, Juan, Yang, Xiaosong, Zhang, Linlin, Wan, Deguang, Wang, Xiangpei, Wu, Hongmei
• Format: Article
• Language: English
• Subjects: Active ingredient; Aquatic plants; Auricle; Biological activity; Chemical composition; Constituents; Ear; Enrichment; Flavones; Flavonoids; Inflammation; Kaempferol; Network pharmacology; Pharmacological studies; Pharmacology; Polygonum capitatum; Proteins; Quercetin; Signal processing; Sugar; Tannins; Xylene
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/632/3/032013

To observe the inhibitory effect of Polygonum capitatum extract on auricle swelling induced by xylene in mice, and explore the mechanism of anti-inflammatory action with the method of network pharmacology. Extract the total flavonoids, sugars and tannins from Polygonum capitatum, and act on xylene induced auricular inflammation in mice. Use 8mm hole punch to lay down the ear piece and measure the quality of the ear piece. Through consulting CNKI, Wanfang, Vip and other databases, the chemical constituents related to Polygonum capitatum were searched. Screening the active chemical constituents of Polygonum capitatum by ADME parameters. Find a target with the active chemical composition and establish a target data set; Protein interaction network (PPI) was used to construct the protein interaction network of target and inflammatory target of Polygonum capitatum. Cytoscape software was used to construct the complex network diagram of "component-target-pathway-disease"; DAVID was used for GO functional enrichment analysis and KEGG pathway enrichment analysis. Result: The experimental results of auricle inflammation induced by xylene in mice showed that the high dose group of total flavones, the low dose group of total flavones and the low dose group of tannin had inhibitory effects on auricle inflammation induced by minor xylene in mice. There were 37 reported compounds, 6 active compounds and 2 flavonoids compounds. A total of 268 targets were retrieved from 6 active compounds, and 41 potential targets were most closely related to the anti-inflammatory mechanism of the Polygonum capitatum.Through the biological function pathway enrichment and KEGG pathway enrichment analysis, 20 biological processes and 76 signal pathways were screened. Conclusion: The anti-inflammatory components of the Polygonum capitatum predicted by the network pharmacology and the experimental results showed that the anti-inflammatory effect of the total flavonoids in the high-dose group was basically the same as that in the low-dose group. It is suggested that Quercetin and Kaempferol may be the anti-inflammatory flavonoids in Polygonum capitatum.