Genetic variability of Citrus spp. in Cibinong germplasm garden using random amplified polymorphic DNA

The preservation and protection of citrus plant germplasms can be done in-situ and ex-situ . Germplasm garden LIPI, Cibinong was built as a local fruit plant garden. The field management also carries out seed propagation and dissemination. This study aims to reveal the genetic diversity of citrus co...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-05, Vol.741 (1), p.12018
Main Authors: Anggraheni, Y G D, Mulyaningsih, E S, Priadi, D
Format: Article
Language:English
Subjects:
Agronomy
Alleles
Biotechnology
Citrus spp
Clustering
Deoxyribonucleic acid
DNA
DNA fingerprinting
Fingerprinting
Fruits
Gardens & gardening
Gene frequency
Genetic analysis
Genetic diversity
Genetic relationship
Genetic variability
Germplasm
Heterozygosity
Plant breeding
Plant diversity
Polymerase chain reaction
Propagation
Random amplified polymorphic DNA
RAPD
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Summary:The preservation and protection of citrus plant germplasms can be done in-situ and ex-situ . Germplasm garden LIPI, Cibinong was built as a local fruit plant garden. The field management also carries out seed propagation and dissemination. This study aims to reveal the genetic diversity of citrus collections and used them as a database for plant breeding and protection activities. This research was conducted at the Agronomy Laboratory for Evaluation of Biotechnology Products-LIPI. Genetic analysis was performed using RAPD techniques, and the resulting data were processed using PowerMarkerver 3.25 and PASTver 3.25 programs . The results showed that seven genetic materials for citrus analyzed with 15 RAPD primers resulted in the mean number of alleles, frequency of main alleles, and heterozygosity are 7.33, 0.49, and 1, respectively. All primers had a PIC value > 0.5, meaning they had high information in fingerprinting research on citrus plant genetic diversity, with an average gene diversity of 0.71. Principal coordinates analysis [PCoA] and clustering obtained two groups with a 0.70 similarity coefficient. Garut and Siam Pontianak have the closest relationship [92% similarity]. On the other hand, the farthest genetic relationship is between Bali and Keprok Batu-55, with a similarity index value of 52%.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/741/1/012018