The effect of medium formulation and BAP concentration of growth and development of Aquilaria malaccensis Lam. shoot in vitro

Tissue culture of agarwood tree ( Aquilaria malaccensis Lam) was done to produce a large number of transplants in the laboratory. The compositions of medium culture are commonly modified to obtain the best growth of explants to regenerate into plantlets. Our study aimed to evaluate and compare diffe...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-05, Vol.762 (1), p.12033
Main Authors: Hapsari, B W, Rudiyanto, Martin, A F, Ermayanti, T M
Format: Article
Language:English
Subjects:
Aquilaria malaccensis
Explants
Leaves
Plantlets
Rooting
Shoots
Tissue culture
Transplants
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Summary:Tissue culture of agarwood tree ( Aquilaria malaccensis Lam) was done to produce a large number of transplants in the laboratory. The compositions of medium culture are commonly modified to obtain the best growth of explants to regenerate into plantlets. Our study aimed to evaluate and compare different medium formulations supplemented with BAP on agarwood shoot culture growth. In this study we used MS, NN, B5, AR and WP medium containing 0 (control treatment), 0.25, 0.5, 1.0 and 2.0 mg/L BAP. The shoot tips were cultured for 16 weeks in the treatment media. Height of shoots, number of leaves, number of adventive shoots, and number of roots was recorded every week to determine the growth. The results showed that after 16 weeks of culture, the best medium for shoot height was NN medium with BAP fortification (3.54cm) for number of leaves and adventive shoots proliferation was Gamborg B5 medium fortified with 0.5 mg/L BAP (21.63 leaves and 8,38 shoots), while NN medium without BAP was best for rooting.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/762/1/012033