Bioelectrochemical processes of oxidation of dicarboxylic amino acids by strain Micrococcus luteus 1-I in a biofuel cell

• Published in: IOP conference series. Earth and environmental science 2021-07, Vol.808 (1), p.12038
• Main Authors: Kuznetsov, A V, Khorina, N N, Konovalova, E Yu, Amsheev, D Yu, Ponamoreva, O N, Stom, D I
• Format: Article
• Language: English
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/808/1/012038

A facultative anaerobic strain was isolated and studied from the activated sludge of the treatment facilities of a petrochemical enterprise. Its morphological and cultural, physiological and biochemical, tinctorial, molecular genetic characteristics have been investigated. Based on the data obtained, strain 1-I was assigned to the species Micrococcus luteus . The electrogenic activity of this bacterium in BFC was shown using dicarboxylic amino acids - glutamic and aspartic. The open-circuit voltage indices in the BFC with M. luteus 1-I increased in 6 days to 511.5 mV with the addition of aspartic acid, and 419 ± 38.5 mV with the addition of glutamic acid. In this case, the short-circuit current increased to 3.17 ± 0.12 and 1.6 ± 0.14 mA, respectively. The specific power of BFCs based on M. luteus 1-I was the highest with the addition of aspartic acid (40-50 mW / m 2 at a current density of 0.15 to 0.4 A / m 2 ). The indicated indicator in a similar BFC with glutamic acid was 26-32 mW / m 2 (at a current density of 0.08 to 0.28 A / m 2 ). The oxidation of these compounds by the studied bacterial strain was also confirmed by the methods of cyclic voltammetry.