Automated Analysis of Human Sperm Number and Concentration (Oligospermia) Using Otsu Threshold Method and Labelling

Oligospermia is a male fertility issue defined as a low sperm concentration in the ejaculate. Normally the sperm concentration is 20-120 million ml, while Oligospermia patients has sperm concentration less than 20 million ml. Sperm test done in the fertility laboratory to determine oligospermia by c...

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Bibliographic Details
Published in:IOP conference series. Materials Science and Engineering 2016-01, Vol.105 (1), p.12038-12047
Main Authors: Susrama, I G, Purnama, K E, Purnomo, M H
Format: Article
Language:English
Subjects:
Automation
Chambers
Concentration
Counting
Fertility
Labelling
Laboratories
Mathematical analysis
Morphology
Oligospermia
Segmentation
Sperm
Threshold
Thresholds
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Summary:Oligospermia is a male fertility issue defined as a low sperm concentration in the ejaculate. Normally the sperm concentration is 20-120 million ml, while Oligospermia patients has sperm concentration less than 20 million ml. Sperm test done in the fertility laboratory to determine oligospermia by checking fresh sperm according to WHO standards in 2010 [9]. The sperm seen in a microscope using a Neubauer improved counting chamber and manually count the number of sperm. In order to be counted automatically, this research made an automation system to analyse and count the sperm concentration called Automated Analysis of Sperm Concentration Counters (A2SC2) using Otsu threshold segmentation process and morphology. Data sperm used is the fresh sperm directly in the analysis in the laboratory from 10 people. The test results using A2SC2 method obtained an accuracy of 91%. Thus in this study, A2SC2 can be used to calculate the amount and concentration of sperm automatically
ISSN:1757-8981
1757-899X
DOI:10.1088/1757-899X/105/1/012038