Application of ellipsometry, spr-technic and raman-spectroscopy into diagnosis of colorectal cancer

Experiments with application of the "Ellips-SPEK" spectral ellipsometric SPR (Surface Plasmon Resonance) analyzer, validating the results on the basis of ProteOn XPR36, revealed the differences in the values of velocity constants of specific interactions of monoclonal highly specific antib...

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Bibliographic Details
Published in:IOP conference series. Materials Science and Engineering 2019-04, Vol.516 (1), p.12017
Main Authors: Kruchinina, M V, Gromov, A A, Prudnikova, Y I, Generalov, V M, Kruchinin, V N, Volodin, V A, Shuvalov, G V, Minin, O V, Shehovtsov, S V, Peltec, S E, Minin, I V
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Biochips
Biomolecules
Blood
Cancer
Diagnosis
Ellipsometry
Metastasis
Pyruvate kinase
Raman spectroscopy
Spectrum analysis
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Summary:Experiments with application of the "Ellips-SPEK" spectral ellipsometric SPR (Surface Plasmon Resonance) analyzer, validating the results on the basis of ProteOn XPR36, revealed the differences in the values of velocity constants of specific interactions of monoclonal highly specific antibodies with tumor M2-pyruvate kinase on the surface of blood serum biochips in patients with various stages colorectal carcinoma (CRC) and location of metastases (AUC 0.89). Scanning ellipsometry enabled to reveal a significant increase of biomolecule surface effective thickness resulting from the specific "antigen-antibody" interaction in the group of patients with extrahepatic and hepatic metastases, as compared to the control group with healthy patients (p
ISSN:1757-8981
1757-899X
1757-899X
DOI:10.1088/1757-899X/516/1/012017