Development of a functional airway-on-a-chip by 3D cell printing

We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolac...

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Bibliographic Details
Published in:Biofabrication 2018-10, Vol.11 (1), p.015002-015002
Main Authors: Park, Ju Young, Ryu, Hyunryul, Lee, Byungjun, Ha, Dong-Heon, Ahn, Minjun, Kim, Suryong, Kim, Jae Yun, Jeon, Noo Li, Cho, Dong-Woo
Format: Article
Language:English
Subjects:
3D cell printing
3D vascular network
airway-on-a-chip
Animals
bioink
Bioprinting - methods
Cell Differentiation
Cell Proliferation
decellularized extracellular matrix
disease modeling
Endothelial Cells - cytology
Extracellular Matrix - chemistry
Extracellular Matrix - metabolism
Fibroblasts - cytology
Humans
Models, Biological
Printing, Three-Dimensional
Swine
Tissue Engineering - standards
Trachea - blood supply
Trachea - cytology
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Summary:We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a lumen and blood vessel network. A fully-differentiated in vitro airway model was assembled with the printed vascular platform, and collectively reproduced a functional interface between the airway epithelium and the vascular network. The model presented respiratory symptoms including asthmatic airway inflammation and allergen-induced asthma exacerbation in physiological context. Because of the adaptable and automated nature of direct 3D cell printing, we expect that this will have relevance in vivo and high reproducibility for production of high-content platforms for preclinical trials in biomedical research.
ISSN:1758-5090
1758-5090
DOI:10.1088/1758-5090/aae545