Enhancement of image quality in planar Airy light-sheet microscopy via subtraction method

The use of propagation-invariant Airy beams enables a light-sheet microscopy with a large field-of-view. Without relying upon two-photon excitation or deconvolution-based processing to eliminate out-of focus blur caused by the side lobes, here, we present how the subtraction method is applied to enh...

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Bibliographic Details
Published in:Journal of optics (2010) 2022-02, Vol.24 (2), p.25301
Main Authors: Deng, Suhui, Yuan, Liusong, Cheng, Peiwei, Wang, Yuhao, Liu, Mingping
Format: Article
Language:English
Subjects:
light-sheet microscopy
planar Airy beam
sectioning ability
subtraction method
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Summary:The use of propagation-invariant Airy beams enables a light-sheet microscopy with a large field-of-view. Without relying upon two-photon excitation or deconvolution-based processing to eliminate out-of focus blur caused by the side lobes, here, we present how the subtraction method is applied to enhance the image quality in digital scanned light-sheet microscopy with Airy beam. In the proposed method, planar Airy beam with the symmetric transversal structure is used to excite the sample. A hollow Airy beam with zero intensity at the focal plane is created, which is mainly used to excite the out-of-focus signal. By scanning the sample twice with the normal planar Airy beam and the hollow Airy beam, digital post-processing of the obtained images by subtraction allows for the rejection of out-of-focus blur and improves the optical sectioning, the axial resolution and the intensity distribution uniformity of the light-sheet microscopy.
ISSN:2040-8978
2040-8986
DOI:10.1088/2040-8986/ac4437