Non-toxic fluorescent phosphonium probes to detect mitochondrial potential

We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characteris...

Full description

Saved in:
Bibliographic Details
Published in:Methods and applications in fluorescence 2017-03, Vol.5 (1), p.015007-015007
Main Authors: Šari, Ana, Crnolatac, Ivo, Bouillaud, Frédéric, Sobo anec, Sandra, Mikecin, Ana-Matea, Ma ak Šafranko, eljka, Delgeorgiev, Todor, Piantanida, Ivo, Balog, Tihomir, Petit, Patrice X
Format: Article
Language:English
Subjects:
confocal microscopy
flow cytometry
membrane potential
mitochondria
probe
respiration
toxicity analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry-xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no-toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes makes them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing-probe's limitations.
ISSN:2050-6120
2050-6120
DOI:10.1088/2050-6120/aa5e64