Examination of the safety of intracytoplasmic injection procedures by using bovine zygotes

We have evaluated the safety of intracytoplasmic sperm injection (ICSI) procedures by using bovine zygotes. Bovine zygotes were injected with a small amount (2–3 pl) of either medium alone or medium containing polyvinylpyrrolidone (PVP) (sham-ICSI, without spermatozoon) using the same procedure as I...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1996-03, Vol.11 (3), p.618-620
Main Authors: Motoishi, Mutsuro, Goto, Kazufumi, Tomita, Keiko, Ookutsu, Shoji, Nakanishi, Yoshihiko
Format: Article
Language:English
Subjects:
Animal productions
Animals
Biological and medical sciences
Birth control
bovine oocyte
Cattle
Culture Media
Cytoplasm
Evaluation Studies as Topic
Female
Fertilization in Vitro - adverse effects
Fertilization in Vitro - methods
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Humans
intracytoplasmic sperm injection
Male
Medical sciences
Microinjections
polyvinylpyrrolidone
Povidone - adverse effects
Safety
Spermatozoa
Sterility. Assisted procreation
Terrestrial animal productions
Vertebrates
Zygote
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Summary:We have evaluated the safety of intracytoplasmic sperm injection (ICSI) procedures by using bovine zygotes. Bovine zygotes were injected with a small amount (2–3 pl) of either medium alone or medium containing polyvinylpyrrolidone (PVP) (sham-ICSI, without spermatozoon) using the same procedure as ICSI, and the subsequent in-vitro embryonic development and embryo quality (number of cells/blasto-cyst) were examined. Control zygotes which had not been injected were similarly evaluated after in-vitro development The sham-ICSI of either medium alone or medium containing PVP into bovine zygotes had no harmful effects on the rate of normal fertilization and on the rate of development to hatched blastocyst stage compared with those of controls (P > 0.05). In addition, no harmful effects were observed in the number of cells per blastocyst (embryo quality). The results suggest, for the first time, that the ICSI procedures currently used for animal and human ICSI are neither detrimental to embryonic development nor detrimental to embryo quality.
ISSN:0268-1161
1460-2350
DOI:10.1093/HUMREP/11.3.618