Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol

The aim of this study was to examine the effect of alcohol on the decidualization of human endometrial stromal cells during early pregnancy. During in vitro decidualization, human endometrial stromal cells were treated with alcohol, 4-methylpyrazole hydrochloride (FPZ), the inhibitor of alcohol dehy...

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Published in:Alcohol and alcoholism (Oxford) 2017-03, Vol.52 (2), p.180
Main Authors: Li, Zi-Long, Li, Meng-Qi, Li, Shu-Yun, Fu, Yong-Sheng, Yang, Zeng-Ming
Format: Article
Language:English
Subjects:
Alcohol Dehydrogenase - biosynthesis
Aldehyde Oxidoreductases - biosynthesis
Apoptosis - drug effects
Apoptosis - physiology
Cell Line, Transformed
Cell Survival - drug effects
Cell Survival - physiology
Decidua - cytology
Decidua - drug effects
Decidua - enzymology
Ethanol - toxicity
Female
Humans
Stromal Cells - cytology
Stromal Cells - drug effects
Stromal Cells - enzymology
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Summary:The aim of this study was to examine the effect of alcohol on the decidualization of human endometrial stromal cells during early pregnancy. During in vitro decidualization, human endometrial stromal cells were treated with alcohol, 4-methylpyrazole hydrochloride (FPZ), the inhibitor of alcohol dehydrogenases (ADHs), and tetraethylthiuram disulfide (DSF), the inhibitor of acetaldehyde dehydrogenases (ALDHs), respectively. Cell viability and decidualization were examined. Apoptosis and proliferation were also evaluated. The findings showed that ADHs and ALDHs were up-regulated during decidualization. After alcohol treatment, the cell viability of decidual stromal cells was significantly higher than control, which was abrogated by FPZ or DSF. When cells were treated with alcohol, proliferation-related signal pathways were up-regulated in decidualized cells. Additionally, FOXO1 transcriptionally up-regulates ADH1B. Our study provided an evidence that highly expressed ADHs and ALDHs endow decidual stromal cells an ability to alleviate the harm from alcohol.
ISSN:0735-0414
1464-3502
DOI:10.1093/alcalc/agw073