Laboratory Evaluation of a Neem Product Against DBM Larvae, 1995

• Published in: Arthropod management tests 1997-01, Vol.22 (1), p.409-409
• Main Authors: Mau, R.F.L., Gusukuma-Minuto, L. R.
• Format: Article
• Language: English
• ISSN: 2155-9856; 2155-9856
• DOI: 10.1093/amt/22.1.409

Treatments were evaluated using the leaf dip method. Head cabbage was seeded in community pots. Each pot containing approximately 10 cabbage plants in the 5 true leaf seedling stage was inverted and dipped in a test insecticide mix for about 30 sees for complete coverage. The dipped plants were allowed to air dry. For each dip, 1 liter insecticide mix was prepared based on field rate concentrations of 100 gal/acre. Leaves from treated plants were detached and placed in a ventilated plastic petri dish. DBM larvae from a laboratory colony that originated from individuals collected from a cabbage field at Kula, Hawaii and Kamuela, Hawaii were used. Ten late 2nd instars were placed on each leaf. Fresh leaves from the original treated plant were added every two days. The number of dead larvae was counted at 24-hour intervals. Larvae were recorded as dead when there was no movement when probed.