Quantitation of follicular non-Hodgkin's lymphoma cells carrying t(14; 18) in a patient before and after allogeneic bone marrow transplantation

A competitive PCR method was developed, enabling accurate quantitation of residual lymphoma cells carrying the t(14; 18) in blood and bone marrow samples of follicular non-Hodgkin's lymphoma (NHL) patients during treatment. A patient with residual lymphoma cells received an allogeneic bone marr...

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Bibliographic Details
Published in:Annals of oncology 1994, Vol.5 (suppl-1), p.S43-S45
Main Authors: Meijerink, J.P.P., Goverde, G.J., Smetsers, T.F.C.M., Raemaekers, J.M.M., Bogman, M.J.J.T., de Witte, T., Mensink, E.J.B.M.
Format: Article
Language:English
Subjects:
18
Adult
Base Sequence
bcl-2
Biological and medical sciences
BMT
Bone Marrow Transplantation
Cell Count
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 18
Hematologic and hematopoietic diseases
Humans
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
lymphoma
Lymphoma, Follicular - genetics
Lymphoma, Follicular - pathology
Lymphoma, Follicular - therapy
Male
Medical sciences
Molecular Sequence Data
PCR
Polymerase Chain Reaction
quantitation
t(14,18)
Translocation, Genetic
Transplantation, Homologous
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Summary:A competitive PCR method was developed, enabling accurate quantitation of residual lymphoma cells carrying the t(14; 18) in blood and bone marrow samples of follicular non-Hodgkin's lymphoma (NHL) patients during treatment. A patient with residual lymphoma cells received an allogeneic bone marrow transplantation (BMT). Since BMT, the patient has been in continuing clinical complete remission. Using the competitive PCR and two-step PCR method, we were able to demonstrate a gradual decline in the number of lymphoma cells within consecutive patient blood and bone marrow samples after BMT. The competitive PCR is a suitable method for the detection of minimal residual disease. Further research might reveal the clinical relevance of data obtained by this method.
ISSN:0923-7534
1569-8041
DOI:10.1093/annonc/5.suppl_1.S43