Blood group antigen expression in developing pancreas and in induced pancreatic cancer cells in Syrian hamsters

Expression of blood grouprelated antigens and the binding pattern of Ulex europaeus-I (UEA-I) in fetal and newborn hamster pancreases was examined immunohistochemically by monoclonal antibodies (MoAbs) against blood group antigens and by peroxidase-conjugated UEA-I. The fetal hamster pancreas was de...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1990-04, Vol.11 (4), p.577-582
Main Authors: Takiyama, Yoshiyuki, Egami, Hiroshi, Pour, Parviz M.
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Animals
Antibodies, Monoclonal
Biological and medical sciences
Blood Group Antigens
Cricetinae
Female
Glycolipids - analysis
Host-tumor relations. Immunology. Biological markers
Immunoenzyme Techniques
Isoantigens
Lectins - metabolism
Lewis X Antigen
Male
Medical sciences
Mesocricetus
Pancreas - cytology
Pancreas - embryology
Pancreas - growth & development
Pancreas - immunology
Pancreatic Neoplasms - chemically induced
Pancreatic Neoplasms - immunology
Plant Lectins
Tumors
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Summary:Expression of blood grouprelated antigens and the binding pattern of Ulex europaeus-I (UEA-I) in fetal and newborn hamster pancreases was examined immunohistochemically by monoclonal antibodies (MoAbs) against blood group antigens and by peroxidase-conjugated UEA-I. The fetal hamster pancreas was detected histologically at the 12th day of gestation. MoAbs A, B and Lea were not immunoreactive with pancreatic cells in any stage of development nor were they after birth. Lex was first expressed in the luminal surface of duct cells at the 13th day of gestation, disappeared at the 15th day of gestation, was re-expressed after birth in acinar cells and was absent in the adult pancreas. Leb and Ley were expressed in acinar cells in late fetal and early newborn stages, but disappeared with maturation and were absent in the adult pancreas. UEA-I binding, however, was demonstrated in both fetal and adult pancreatic tissue. In cancer cells induced by BOP, bloud group antigens except for Lea and UEA-I binding were found in various reactivity. These findings suggest that in hamsters (i) A and B antigens are tumor-related antigens; (ii) H, Leb, Lex and Ley are oncofetal antigens; and (iii) fucosylation is an important event in cell differentiation.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/11.4.577