Experimental colon carcinogenesis is facilitated by endogenous factors in the intestinal contents

The theory that endogenous factors in the intestinal contents may be pathogenic during large bowel carcinogenesis was tested in the dimethylhydrazine (DMH)-induced rat colon cancer model. Thirty female Wistar rats, each serving as their own control, had a surgical transection of the proximal colon w...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1981, Vol.2 (12), p.1363-1366
Main Authors: Lewin, M.R., Ferulano, G.P., Cruse, J.P., Clark, C.G.
Format: Article
Language:English
Subjects:
Adenoma - etiology
Adenoma - pathology
Animals
Colon - surgery
Colonic Neoplasms - chemically induced
Colonic Neoplasms - etiology
Colonic Neoplasms - pathology
Dimethylhydrazines
Feces
Female
Neoplasms, Experimental - etiology
Neoplasms, Experimental - pathology
Rats
Rats, Inbred Strains
Rectum - surgery
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Summary:The theory that endogenous factors in the intestinal contents may be pathogenic during large bowel carcinogenesis was tested in the dimethylhydrazine (DMH)-induced rat colon cancer model. Thirty female Wistar rats, each serving as their own control, had a surgical transection of the proximal colon with reanastomosis to the rectum, thereby excluding part of the colon from faecal contact. All rats then received a course of DMH (40 mg/kg body wt/wk s.c. for 10 weeks) while fed on Vivonex. This diet was selected because it lacks known exogenous (dietary) cocarcinogens. It also produces mucosal atrophy in functioning (proximal) colon, to parallel the disuse atrophy induced in the defunctioned (distal) colon. Animals remained on the diet throughout the experiment and were killed when moribund or at 40 weeks. At necropsy, the anatomical distribution, number, size and histological type of colon tumours were compared between functioning and defunctioned colonic segments within the same animal. At autopsy, there were significantly fewer colon tumours in the defunctioned segment (P < 0.005). Furthermore, there were significantly fewer carcinomas (P 1 cm diameter (P
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/2.12.1363