Folic Acid Inhibits Aging-Induced Telomere Attrition and Apoptosis in Astrocytes In Vivo and In Vitro

• Published in: Cerebral cortex (New York, N.Y. 1991) N.Y. 1991), 2022-01, Vol.32 (2), p.286-297
• Main Authors: Li, Zhenshu, Zhou, Dezheng, Zhang, Dalong, Zhao, Jing, Li, Wen, Sun, Yue, Chen, Yongjie, Liu, Huan, Wilson, John X, Qian, Zhiyong, Huang, Guowei
• Format: Article
• Language: English
• Subjects: Aging; Animals; Apoptosis; Astrocytes; Folic Acid - pharmacology; Mice; Telomere
• ISSN: 1047-3211; 1460-2199
• DOI: 10.1093/cercor/bhab208

Abstract Folic acid (FA) has been reported to inhibit astrocyte apoptosis and improve aging-induced disorders; however, its role in telomere attrition remains unclear. In present study, 4-month-old senescence-accelerated mouse prone 8 (SAMP8) mice were assigned to four treatment groups for the in vivo experiment: FA-deficient diet (FA-D) group, FA-normal diet (FA-N) group, low FA-supplemented diet (FA-L) group, and high FA-supplemented diet (FA-H) group. These mice were euthanized when 10 months old. There was also a young SAMP8 (4 months old) control group (Con-Y) fed with FA-normal diet. In in vitro study, primary cultures of astrocytes from hippocampus and cerebral cortex were incubated for five generations with various concentrations of FA (0–40 μM) and were assigned to five groups: FA 0 μM (generation 5), FA 10 μM (generation 5), FA 20 μM (generation 5), FA 40 μM (generation 5), and FA 10 μM (generation 1). The results showed that FA supplementation inhibited aging-induced astrocytosis, astrocyte apoptosis, neurodegeneration, and prevented telomere attrition in hippocampus and cortex of SAMP8 mice. FA supplementation also decreased apoptosis and telomere attrition, and increased telomerase activity, in primary cultures of astrocytes. These results showed that it may be one of the mechanisms that FA inhibiting aging-induced apoptosis of astrocyte by alleviating telomere attrition.