Co-expression of anoctamins in cilia of olfactory sensory neurons

Vertebrates can sense and identify a vast array of chemical cues. The molecular machinery involved in chemodetection and transduction is expressed within the cilia of olfactory sensory neurons. Currently, there is only limited information available on the distribution and density of individual signa...

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Bibliographic Details
Published in:Chemical senses 2015-02, Vol.40 (2), p.73-87
Main Authors: Henkel, Bastian, Drose, Daniela R, Ackels, Tobias, Oberland, Sonja, Spehr, Marc, Neuhaus, Eva M
Format: Article
Language:English
Subjects:
Animals
Anoctamins
Chloride Channels - genetics
Chloride Channels - metabolism
Cilia - metabolism
Cyclic Nucleotide-Gated Cation Channels - metabolism
Gene Expression Regulation
HEK293 Cells
Humans
Mice, Inbred C57BL
Olfactory Mucosa - cytology
Olfactory Mucosa - metabolism
Olfactory Receptor Neurons - metabolism
Phospholipid Transfer Proteins - genetics
Phospholipid Transfer Proteins - metabolism
Protein Multimerization
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Sensory Receptor Cells - metabolism
Signal Transduction
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Summary:Vertebrates can sense and identify a vast array of chemical cues. The molecular machinery involved in chemodetection and transduction is expressed within the cilia of olfactory sensory neurons. Currently, there is only limited information available on the distribution and density of individual signaling components within the ciliary compartment. Using super-resolution microscopy, we show here that cyclic-nucleotide-gated channels and calcium-activated chloride channels of the anoctamin family are localized to discrete microdomains in the ciliary membrane. In addition to ANO2, a second anoctamin, ANO6, also localizes to ciliary microdomains. This observation, together with the fact that ANO6 and ANO2 co-localize, indicates a role for ANO6 in olfactory signaling. We show that both ANO2 and ANO6 can form heteromultimers and that this heteromerization alters the recombinant channels' physiological properties. Thus, we provide evidence for interaction of ANO2 and ANO6 in olfactory cilia, with possible physiological relevance for olfactory signaling.
ISSN:0379-864X
1464-3553
DOI:10.1093/chemse/bju061