Solid-Phase Extraction and Cleanup of Organophosphorus Pesticide Residues in Bovine Muscle with Gas Chromatographic Detection

• Published in: Journal of chromatographic science 2002-08, Vol.40 (7), p.392-396
• Main Authors: Kuivinen, Jorma, Bengtsson, Staffan
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cattle; Chromatography, Gas - methods; Food industries; Fundamental and applied biological sciences. Psychology; Insecticides - analysis; Meat and meat product industries; Organophosphorus Compounds; Pesticide Residues - analysis; Reference Standards; Sensitivity and Specificity
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/40.7.392

The purpose of this investigation is to develop a rapid and simple method for the assessment of organophosphorus pesticides in bovine muscle by using solid-phase extraction (SPE). After extraction with ethyl acetate (EtOAc) the homogenate is centrifuged and filtered through sodium sulfate. The fat is precipitated in methanol by cooling and the extract is diluted with water and passed through a SPE column (Isolute ENV+). After elution with EtOAc, evaporation, and redissolution, the sample is injected into a gas chromatographic (GC) capillary column DB-1701 and detected by a flame photometric detector. Recoveries from bovine muscle fortified with 12 pesticides between 4 and 65 µg/kg include three levels ranging between 59% and 109% for ten of them. The two most polar pesticides (metamidophos and acephate) are not successful. The relative standard deviations are between 1% and 10% for the ten pesticides. A simplex method is used to optimize the GC conditions.