Sandwich-type enzyme immunoassay for big endothelin-1 in plasma: concentrations in healthy human subjects unaffected by sex or posture

A sandwich-type enzyme immunoassay has been developed for measuring human big endothelin-1 (big ET-1) in human plasma and supernatant fluids from human cell cultures. Big ET-1 is the precursor of endothelin 1 (ET-1), the most potent vasoconstrictor known. A rabbit antibody raised against the big ET-...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1997-01, Vol.43 (1), p.64-70
Main Authors: Aubin, Philippe, Le Brun, Gaelle, Moldovan, Florina, Villette, Jean-Marie, Creminon, Christophe, Dumas, Jacques, Homyrda, Laurence, Soliman, Hany, Azizi, Michel, Fiet, Jean
Format: Article
Language:English
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Summary:A sandwich-type enzyme immunoassay has been developed for measuring human big endothelin-1 (big ET-1) in human plasma and supernatant fluids from human cell cultures. Big ET-1 is the precursor of endothelin 1 (ET-1), the most potent vasoconstrictor known. A rabbit antibody raised against the big ET-1 COOH-terminus fragment was used as an immobilized antibody (anti-P16). The Fab′ fragment of a monoclonal antibody (1B3) raised against the ET-1 loop fragment was used as the enzyme-labeled antibody, after being coupled to acetylcholinesterase. The lowest detectable value in the assay was 1.2 pg/mL (0.12 pg/well). The assay was highly specific for big ET-1, demonstrating no cross-reactivity with ET-1,
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/43.1.64