Development of an enzyme-linked immunosorbent assay with monoclonal antibody for quantification of homovanillic in human urine samples

A monoclonal antibody to homovanillic acid (HVA) was prepared by synthesis of a HVA-protein conjugate (HVA-ovalbumin) as an immunogen, immunization of mice, and the subsequent hybridization technique. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and accuracy. An indi...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1998-08, Vol.44 (8), p.1674-1679
Main Authors: Shi, Run Zhang, Ho, Yee-Ping, Yeung, John Hok Keung, Or, Penelope Mei Yu, To, Kenneth Kin Wah, Lau, Mary Wai Man, Arumanayagam, Mano
Format: Article
Language:English
Subjects:
Biological and medical sciences
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Nervous system
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
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Summary:A monoclonal antibody to homovanillic acid (HVA) was prepared by synthesis of a HVA-protein conjugate (HVA-ovalbumin) as an immunogen, immunization of mice, and the subsequent hybridization technique. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and accuracy. An indirect ELISA was developed for quantification of HVA in human urine. The assay was characterized and shown to have high specificity, with cross-reactivities to vanillylmandelic acid and normetanephrine at 0.18% and
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/44.8.1674