Severe neutrophil depletion by leucocyte filters or cytotoxic drug does not improve recovery of contractile function in stunned porcine myocardium

Objective: The contribution of neutrophils to myocardial injury during stunning remains controversial because of conflicting results in neutropenic animals. The goal of this study was to compare the recovery of function in stunned myocardium using two distinct methods for inducing neutropenia in pig...

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Bibliographic Details
Published in:Cardiovascular research 1993-05, Vol.27 (5), p.720-727
Main Authors: Juneau, Carl F, Ito, Bruce R, Balzo, Ughetta del, Engler, Robert L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiology. Vascular system
Cardiopathies: etiologic forms (general aspects and miscellaneous)
Cyclophosphamide - pharmacology
Disease Models, Animal
Female
free radicals
Heart
leucocyte filters
Leukocyte Count
Male
Medical sciences
Micropore Filters
Myocardial Contraction - physiology
Myocardial Reperfusion Injury - etiology
Myocardial Reperfusion Injury - pathology
myocardial stunning
Neutropenia - chemically induced
neutrophils
Neutrophils - pathology
Neutrophils - physiology
Swine
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Summary:Objective: The contribution of neutrophils to myocardial injury during stunning remains controversial because of conflicting results in neutropenic animals. The goal of this study was to compare the recovery of function in stunned myocardium using two distinct methods for inducing neutropenia in pigs. Methods: Three groups of pigs were studied: control (n=6), and made neutropenic by either Leukopak™ blood filters (n=7), or cyclophosphamide (n=7, 50 mg·kg−1 intravenously 4 d prior to study). In anaesthetised open chest pigs, with the heart paced at 110 beats·min−1, the left anterior descending coronary artery was perfused with an extracorporeal circuit at controlled coronary pressure (CP) and the regional coronary blood flow was measured. Systolic wall thickening was determined by sonomicrometry in the left anterior descending and circumflex coronary artery regions. The protocol consisted of 15 min of low flow ischaemia (CP=40 mm Hg), followed by a staged reperfusion over 10 min back to baseline (CP=90 mm Hg), and continued for 2 h. Blood filtration was initiated prior to ischaemia and stopped after 90 min reperfusion. Results: In both treated groups during ischaemia and the initial 60 min of reperfusion the neutrophil count was severely depleted to
ISSN:0008-6363
1755-3245
DOI:10.1093/cvr/27.5.720