Loading…

P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis

Abstract Background The oral microbiome in ulcerative colitis (UC) patients and its role in the pathogenesis of the disease are still poorly understood. Although there are studies investigating the composition of oral microbiome in UC, there are no studies on concomitant analyses of oral and colonic...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Crohn's and colitis 2024-01, Vol.18 (Supplement_1), p.i2174-i2174
Main Authors: Karakan, T, Özmeriç Kurtuluş, N, Ünsal, B, Çağrı İşler, S, Akça, G, Soysal, F, Cindoruk, M, Elgün Ulkar, S, Açık, L, Ergin, M, Abbasov, Z, Özkul Koçak, C, Ekmen, N, Yalınay, A M, Özkan, S, Karataş, A, Paes Batista Da Silva, A
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page i2174
container_issue Supplement_1
container_start_page i2174
container_title Journal of Crohn's and colitis
container_volume 18
creator Karakan, T
Özmeriç Kurtuluş, N
Ünsal, B
Çağrı İşler, S
Akça, G
Soysal, F
Cindoruk, M
Elgün Ulkar, S
Açık, L
Ergin, M
Abbasov, Z
Özkul Koçak, C
Ekmen, N
Yalınay, A M
Özkan, S
Karataş, A
Paes Batista Da Silva, A
description Abstract Background The oral microbiome in ulcerative colitis (UC) patients and its role in the pathogenesis of the disease are still poorly understood. Although there are studies investigating the composition of oral microbiome in UC, there are no studies on concomitant analyses of oral and colonic mucosal samples in treatment-naïve patients. In this study, the oral and mucosal gut microbiome of UC patients and oral microbiota of healthy individuals (HC) were compared. Methods Sixty patients with newly-diagnosed active (mild-moderate severity) UC patients (n=30) and HC (n=30) were included in the study. Dietary habits were stable in the last three months of participants (checked by dietary recall questionnaires) and co-morbidities affecting microbiota profiles were excluded. Multiple colonic mucosa biopsies were obtained from inflamed areas before treatment in UC group. Simultaneously, subgingival plaque samples were analyzed. All samples were identified through next-generation DNA sequencing analysis, evaluated using bioinformatic tests. Results The potential signature bacterial species associated with UC were determined by examining of both gut and oral microbiomes. According to the Microbiome Lefse analysis, Prevotella copri emerged as the prominent common species in the colonic mucosal and subgingival plaque biopsies of UC group. Oral microbiome comparison between UC and HC patients revealed increased Haemophilus parainfluenza and Corynebacterium durum species and decreased F. prastnutzii and Akkermansia muciniphilia in the UC group (p
doi_str_mv 10.1093/ecco-jcc/jjad212.1356
format article
fullrecord <record><control><sourceid>oup_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1093_ecco_jcc_jjad212_1356</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/ecco-jcc/jjad212.1356</oup_id><sourcerecordid>10.1093/ecco-jcc/jjad212.1356</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1346-37cdbd0b77b704b44db9d9267093bfeb648e6ce5ffffbe08c6da754dcfea4f843</originalsourceid><addsrcrecordid>eNptkEtOwzAQQC0EEqVwBCRfwK2dOHayROUrVYIFrCN_wVESV7YD4lQcgovh0O5gNjMjzRvNPAAuCV4R3JRro5RHnVLrrhO6IMWKlBU7AgtSc4Yo5c3xb12ipqHsFJzF2GFcNRWvFyA9kaJg8NokEwY3iuT8CL2FPogeilHD1ynBYVI-5n5wKnjp_GDgLnjrehPn2V2mzJgi_HDpDaZgRBpyj0bx_fVu4NQrE_JILpXvXXLxHJxY0UdzcchL8HJ787y5R9vHu4fN1RYpUlKGSq601FhyLjmmklItG90UjOenpTWS0dowZSqbQxpcK6YFr6hW1ghqa1ouQbXfm8-OMRjb7oIbRPhsCW5nde2srs3q2oO6dlaXObzn_LT7F0F_kB9jVXnO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis</title><source>Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)</source><creator>Karakan, T ; Özmeriç Kurtuluş, N ; Ünsal, B ; Çağrı İşler, S ; Akça, G ; Soysal, F ; Cindoruk, M ; Elgün Ulkar, S ; Açık, L ; Ergin, M ; Abbasov, Z ; Özkul Koçak, C ; Ekmen, N ; Yalınay, A M ; Özkan, S ; Karataş, A ; Paes Batista Da Silva, A</creator><creatorcontrib>Karakan, T ; Özmeriç Kurtuluş, N ; Ünsal, B ; Çağrı İşler, S ; Akça, G ; Soysal, F ; Cindoruk, M ; Elgün Ulkar, S ; Açık, L ; Ergin, M ; Abbasov, Z ; Özkul Koçak, C ; Ekmen, N ; Yalınay, A M ; Özkan, S ; Karataş, A ; Paes Batista Da Silva, A</creatorcontrib><description>Abstract Background The oral microbiome in ulcerative colitis (UC) patients and its role in the pathogenesis of the disease are still poorly understood. Although there are studies investigating the composition of oral microbiome in UC, there are no studies on concomitant analyses of oral and colonic mucosal samples in treatment-naïve patients. In this study, the oral and mucosal gut microbiome of UC patients and oral microbiota of healthy individuals (HC) were compared. Methods Sixty patients with newly-diagnosed active (mild-moderate severity) UC patients (n=30) and HC (n=30) were included in the study. Dietary habits were stable in the last three months of participants (checked by dietary recall questionnaires) and co-morbidities affecting microbiota profiles were excluded. Multiple colonic mucosa biopsies were obtained from inflamed areas before treatment in UC group. Simultaneously, subgingival plaque samples were analyzed. All samples were identified through next-generation DNA sequencing analysis, evaluated using bioinformatic tests. Results The potential signature bacterial species associated with UC were determined by examining of both gut and oral microbiomes. According to the Microbiome Lefse analysis, Prevotella copri emerged as the prominent common species in the colonic mucosal and subgingival plaque biopsies of UC group. Oral microbiome comparison between UC and HC patients revealed increased Haemophilus parainfluenza and Corynebacterium durum species and decreased F. prastnutzii and Akkermansia muciniphilia in the UC group (p&lt;0.022). Comparative Boxplot analysis of bacterial abundance and alpha-diversity, as indicated by the Shannon index data, revealed that the HC group exhibited higher bacterial abundance and diversity in subgingival plaque samples than the UC group. We found significant association of Bacteroides vulgatus, Prevotella copri, Bacteroides fragilis, Parabacteroides merdae in both colonic mucosal and subgingival plaque samples (oral microbiome) of UC patients (p&lt;0.044). Conclusion Our study is the first to show the oral and colonic mucosal microbiome relationship in UC. The presence of oral dysbiosis associated with UC in our study supports the hypothesis that UC could be associated not only with gut dysbiosis, as previously observed, but also with an imbalance in the oral microbial communities. Interestingly, we could not detect previously reported species of Streptococcus, Oribacterium, Rothia, Neisseria and Porphyromonas in the oral microbiome samples of the UC group. However, oral and gut microbiome profiles share some common microorganisms such as Prevotella copri and Bacteroides fragilis. Further studies are required for determining the potential role of oral dysbiosis is disease severity.</description><identifier>ISSN: 1873-9946</identifier><identifier>EISSN: 1876-4479</identifier><identifier>DOI: 10.1093/ecco-jcc/jjad212.1356</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><ispartof>Journal of Crohn's and colitis, 2024-01, Vol.18 (Supplement_1), p.i2174-i2174</ispartof><rights>The Author(s) 2024. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. All rights reserved. For permissions, please email: journals.permissions@oup.com 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Karakan, T</creatorcontrib><creatorcontrib>Özmeriç Kurtuluş, N</creatorcontrib><creatorcontrib>Ünsal, B</creatorcontrib><creatorcontrib>Çağrı İşler, S</creatorcontrib><creatorcontrib>Akça, G</creatorcontrib><creatorcontrib>Soysal, F</creatorcontrib><creatorcontrib>Cindoruk, M</creatorcontrib><creatorcontrib>Elgün Ulkar, S</creatorcontrib><creatorcontrib>Açık, L</creatorcontrib><creatorcontrib>Ergin, M</creatorcontrib><creatorcontrib>Abbasov, Z</creatorcontrib><creatorcontrib>Özkul Koçak, C</creatorcontrib><creatorcontrib>Ekmen, N</creatorcontrib><creatorcontrib>Yalınay, A M</creatorcontrib><creatorcontrib>Özkan, S</creatorcontrib><creatorcontrib>Karataş, A</creatorcontrib><creatorcontrib>Paes Batista Da Silva, A</creatorcontrib><title>P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis</title><title>Journal of Crohn's and colitis</title><description>Abstract Background The oral microbiome in ulcerative colitis (UC) patients and its role in the pathogenesis of the disease are still poorly understood. Although there are studies investigating the composition of oral microbiome in UC, there are no studies on concomitant analyses of oral and colonic mucosal samples in treatment-naïve patients. In this study, the oral and mucosal gut microbiome of UC patients and oral microbiota of healthy individuals (HC) were compared. Methods Sixty patients with newly-diagnosed active (mild-moderate severity) UC patients (n=30) and HC (n=30) were included in the study. Dietary habits were stable in the last three months of participants (checked by dietary recall questionnaires) and co-morbidities affecting microbiota profiles were excluded. Multiple colonic mucosa biopsies were obtained from inflamed areas before treatment in UC group. Simultaneously, subgingival plaque samples were analyzed. All samples were identified through next-generation DNA sequencing analysis, evaluated using bioinformatic tests. Results The potential signature bacterial species associated with UC were determined by examining of both gut and oral microbiomes. According to the Microbiome Lefse analysis, Prevotella copri emerged as the prominent common species in the colonic mucosal and subgingival plaque biopsies of UC group. Oral microbiome comparison between UC and HC patients revealed increased Haemophilus parainfluenza and Corynebacterium durum species and decreased F. prastnutzii and Akkermansia muciniphilia in the UC group (p&lt;0.022). Comparative Boxplot analysis of bacterial abundance and alpha-diversity, as indicated by the Shannon index data, revealed that the HC group exhibited higher bacterial abundance and diversity in subgingival plaque samples than the UC group. We found significant association of Bacteroides vulgatus, Prevotella copri, Bacteroides fragilis, Parabacteroides merdae in both colonic mucosal and subgingival plaque samples (oral microbiome) of UC patients (p&lt;0.044). Conclusion Our study is the first to show the oral and colonic mucosal microbiome relationship in UC. The presence of oral dysbiosis associated with UC in our study supports the hypothesis that UC could be associated not only with gut dysbiosis, as previously observed, but also with an imbalance in the oral microbial communities. Interestingly, we could not detect previously reported species of Streptococcus, Oribacterium, Rothia, Neisseria and Porphyromonas in the oral microbiome samples of the UC group. However, oral and gut microbiome profiles share some common microorganisms such as Prevotella copri and Bacteroides fragilis. Further studies are required for determining the potential role of oral dysbiosis is disease severity.</description><issn>1873-9946</issn><issn>1876-4479</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptkEtOwzAQQC0EEqVwBCRfwK2dOHayROUrVYIFrCN_wVESV7YD4lQcgovh0O5gNjMjzRvNPAAuCV4R3JRro5RHnVLrrhO6IMWKlBU7AgtSc4Yo5c3xb12ipqHsFJzF2GFcNRWvFyA9kaJg8NokEwY3iuT8CL2FPogeilHD1ynBYVI-5n5wKnjp_GDgLnjrehPn2V2mzJgi_HDpDaZgRBpyj0bx_fVu4NQrE_JILpXvXXLxHJxY0UdzcchL8HJ787y5R9vHu4fN1RYpUlKGSq601FhyLjmmklItG90UjOenpTWS0dowZSqbQxpcK6YFr6hW1ghqa1ouQbXfm8-OMRjb7oIbRPhsCW5nde2srs3q2oO6dlaXObzn_LT7F0F_kB9jVXnO</recordid><startdate>20240124</startdate><enddate>20240124</enddate><creator>Karakan, T</creator><creator>Özmeriç Kurtuluş, N</creator><creator>Ünsal, B</creator><creator>Çağrı İşler, S</creator><creator>Akça, G</creator><creator>Soysal, F</creator><creator>Cindoruk, M</creator><creator>Elgün Ulkar, S</creator><creator>Açık, L</creator><creator>Ergin, M</creator><creator>Abbasov, Z</creator><creator>Özkul Koçak, C</creator><creator>Ekmen, N</creator><creator>Yalınay, A M</creator><creator>Özkan, S</creator><creator>Karataş, A</creator><creator>Paes Batista Da Silva, A</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20240124</creationdate><title>P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis</title><author>Karakan, T ; Özmeriç Kurtuluş, N ; Ünsal, B ; Çağrı İşler, S ; Akça, G ; Soysal, F ; Cindoruk, M ; Elgün Ulkar, S ; Açık, L ; Ergin, M ; Abbasov, Z ; Özkul Koçak, C ; Ekmen, N ; Yalınay, A M ; Özkan, S ; Karataş, A ; Paes Batista Da Silva, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1346-37cdbd0b77b704b44db9d9267093bfeb648e6ce5ffffbe08c6da754dcfea4f843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karakan, T</creatorcontrib><creatorcontrib>Özmeriç Kurtuluş, N</creatorcontrib><creatorcontrib>Ünsal, B</creatorcontrib><creatorcontrib>Çağrı İşler, S</creatorcontrib><creatorcontrib>Akça, G</creatorcontrib><creatorcontrib>Soysal, F</creatorcontrib><creatorcontrib>Cindoruk, M</creatorcontrib><creatorcontrib>Elgün Ulkar, S</creatorcontrib><creatorcontrib>Açık, L</creatorcontrib><creatorcontrib>Ergin, M</creatorcontrib><creatorcontrib>Abbasov, Z</creatorcontrib><creatorcontrib>Özkul Koçak, C</creatorcontrib><creatorcontrib>Ekmen, N</creatorcontrib><creatorcontrib>Yalınay, A M</creatorcontrib><creatorcontrib>Özkan, S</creatorcontrib><creatorcontrib>Karataş, A</creatorcontrib><creatorcontrib>Paes Batista Da Silva, A</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Crohn's and colitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karakan, T</au><au>Özmeriç Kurtuluş, N</au><au>Ünsal, B</au><au>Çağrı İşler, S</au><au>Akça, G</au><au>Soysal, F</au><au>Cindoruk, M</au><au>Elgün Ulkar, S</au><au>Açık, L</au><au>Ergin, M</au><au>Abbasov, Z</au><au>Özkul Koçak, C</au><au>Ekmen, N</au><au>Yalınay, A M</au><au>Özkan, S</au><au>Karataş, A</au><au>Paes Batista Da Silva, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis</atitle><jtitle>Journal of Crohn's and colitis</jtitle><date>2024-01-24</date><risdate>2024</risdate><volume>18</volume><issue>Supplement_1</issue><spage>i2174</spage><epage>i2174</epage><pages>i2174-i2174</pages><issn>1873-9946</issn><eissn>1876-4479</eissn><abstract>Abstract Background The oral microbiome in ulcerative colitis (UC) patients and its role in the pathogenesis of the disease are still poorly understood. Although there are studies investigating the composition of oral microbiome in UC, there are no studies on concomitant analyses of oral and colonic mucosal samples in treatment-naïve patients. In this study, the oral and mucosal gut microbiome of UC patients and oral microbiota of healthy individuals (HC) were compared. Methods Sixty patients with newly-diagnosed active (mild-moderate severity) UC patients (n=30) and HC (n=30) were included in the study. Dietary habits were stable in the last three months of participants (checked by dietary recall questionnaires) and co-morbidities affecting microbiota profiles were excluded. Multiple colonic mucosa biopsies were obtained from inflamed areas before treatment in UC group. Simultaneously, subgingival plaque samples were analyzed. All samples were identified through next-generation DNA sequencing analysis, evaluated using bioinformatic tests. Results The potential signature bacterial species associated with UC were determined by examining of both gut and oral microbiomes. According to the Microbiome Lefse analysis, Prevotella copri emerged as the prominent common species in the colonic mucosal and subgingival plaque biopsies of UC group. Oral microbiome comparison between UC and HC patients revealed increased Haemophilus parainfluenza and Corynebacterium durum species and decreased F. prastnutzii and Akkermansia muciniphilia in the UC group (p&lt;0.022). Comparative Boxplot analysis of bacterial abundance and alpha-diversity, as indicated by the Shannon index data, revealed that the HC group exhibited higher bacterial abundance and diversity in subgingival plaque samples than the UC group. We found significant association of Bacteroides vulgatus, Prevotella copri, Bacteroides fragilis, Parabacteroides merdae in both colonic mucosal and subgingival plaque samples (oral microbiome) of UC patients (p&lt;0.044). Conclusion Our study is the first to show the oral and colonic mucosal microbiome relationship in UC. The presence of oral dysbiosis associated with UC in our study supports the hypothesis that UC could be associated not only with gut dysbiosis, as previously observed, but also with an imbalance in the oral microbial communities. Interestingly, we could not detect previously reported species of Streptococcus, Oribacterium, Rothia, Neisseria and Porphyromonas in the oral microbiome samples of the UC group. However, oral and gut microbiome profiles share some common microorganisms such as Prevotella copri and Bacteroides fragilis. Further studies are required for determining the potential role of oral dysbiosis is disease severity.</abstract><cop>UK</cop><pub>Oxford University Press</pub><doi>10.1093/ecco-jcc/jjad212.1356</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1873-9946
ispartof Journal of Crohn's and colitis, 2024-01, Vol.18 (Supplement_1), p.i2174-i2174
issn 1873-9946
1876-4479
language eng
recordid cdi_crossref_primary_10_1093_ecco_jcc_jjad212_1356
source Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)
title P1226 Determination of oral and gut mucosal microbiome profiles of patients with treatment-naïve ulcerative colitis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T06%3A02%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-oup_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=P1226%20Determination%20of%20oral%20and%20gut%20mucosal%20microbiome%20profiles%20of%20patients%20with%20treatment-na%C3%AFve%20ulcerative%20colitis&rft.jtitle=Journal%20of%20Crohn's%20and%20colitis&rft.au=Karakan,%20T&rft.date=2024-01-24&rft.volume=18&rft.issue=Supplement_1&rft.spage=i2174&rft.epage=i2174&rft.pages=i2174-i2174&rft.issn=1873-9946&rft.eissn=1876-4479&rft_id=info:doi/10.1093/ecco-jcc/jjad212.1356&rft_dat=%3Coup_cross%3E10.1093/ecco-jcc/jjad212.1356%3C/oup_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c1346-37cdbd0b77b704b44db9d9267093bfeb648e6ce5ffffbe08c6da754dcfea4f843%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_oup_id=10.1093/ecco-jcc/jjad212.1356&rfr_iscdi=true