Heterogeneity of Enteroaggregative Escherichia Coli Virulence Demonstrated

Enteroaggregative Escherichia coli (EAggEC) are diarrheal pathogens defined by aggregative adherence to HEp-2 cells. In an effort to identify pathogenic EAggEC isolates, four groups of 5 volunteers were fed 1 of 4 different EAggEC strains, each at a dose of 1010 cfu. Strain 042 caused diarrhea in 3...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-02, Vol.171 (2), p.465-468
Main Authors: Nataro, James P., Yikang, Deng, Cookson, Susan, Cravioto, Alejandro, Savarino, Stephen J., Guers, Linda D., Levine, Myron M., Tacket, Carol O.
Format: Article
Language:English
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Summary:Enteroaggregative Escherichia coli (EAggEC) are diarrheal pathogens defined by aggregative adherence to HEp-2 cells. In an effort to identify pathogenic EAggEC isolates, four groups of 5 volunteers were fed 1 of 4 different EAggEC strains, each at a dose of 1010 cfu. Strain 042 caused diarrhea in 3 of 5 adults; 3 other EAggEC isolates (17–2, 34b, and JM221) failed to elicit diarrhea. A gene encoding enterotoxin EASTI was found in strains 042 and 17–2 but not 34b or JM221; a 108-kDa cytotoxin was expressed in all 4 isolates. All 4 isolates showed a modest degree of gentamicin protection in HEp-2 cells. 17–2, 34b, and JM221 expressed the fimbrial antigen AAF/I; 042 did not express this fimbria as determined by immunogold electron microscopy and genetic probe hybridization.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/171.2.465