Cobas Mira™ S Endpoint Enzymatic Assay for Plasma Formate

In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented....

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Bibliographic Details
Published in:Journal of analytical toxicology 2001-03, Vol.25 (2), p.77-80
Main Authors: Blomme, B., Lheureux, P., Gerlo, E., Maes, V.
Format: Article
Language:English
Subjects:
Acidosis - diagnosis
Acidosis - metabolism
Adult
Formate Dehydrogenases - metabolism
Formates - blood
Humans
Indicators and Reagents
Male
Methanol - metabolism
Methanol - toxicity
NAD - metabolism
Reproducibility of Results
Sensitivity and Specificity
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Description
Summary:In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of sample is incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide. Fifteen microliters of a suspension of formate dehydrogenase is then added. Absorbance at 340 nm is measured every 25 s. The NADH produced when formate is oxidized is stoichiometric to the amount of formate. The method is sensitive, reproducible, and specific and has a broad measurement range. The frozen reagents are stable for at least six months, so the described method can be applied to irregular and semi-urgent requests. A recent case is reported.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/25.2.77