Quantitation of Barbiturates by a Modification of the EMIT®-tox™ Serum Barbiturate Assay

A modification of the Syva EMIT-tox Serum Barbiturate Assay was developed to provide a rapid and practical method for quantitating serum barbiturates. Standard curves for eight selected barbiturates were run using specific sets of standards for each. Correlation coefficients, slopes, and y-intercept...

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Bibliographic Details
Published in:Journal of analytical toxicology 1983-11, Vol.7 (6), p.257-261
Main Authors: Watson, Ann T., Manno, Joseph E., Manno, Barbara R.
Format: Article
Language:English
Subjects:
Antibody Specificity
Barbiturates - blood
Barbiturates - poisoning
Humans
Immunoenzyme Techniques
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Summary:A modification of the Syva EMIT-tox Serum Barbiturate Assay was developed to provide a rapid and practical method for quantitating serum barbiturates. Standard curves for eight selected barbiturates were run using specific sets of standards for each. Correlation coefficients, slopes, and y-intercepts for the curves of best fit were determined by the least-squares method. Between-run and within-run precision analysis indicated acceptable reproducibility for barbiturate concentrations in high therapeutic to toxic serum levels. A case report describing the use of this method for the determination of serum pentobarbital concentrations during therapy for intracranial hypertension is presented. The modified EMIT assay may be useful for monitoring barbiturate blood levels in overdose, and when used for therapy of ischemic brain damage.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/7.6.257