Quantitation of Diethylene Glycol and Its Metabolites by Gas Chromatography Mass Spectrometry or Ion Chromatography Mass Spectrometry in Rat and Human Biological Samples

The misuse of the commonly used chemical diethylene glycol (DEG) has lead to many poisonings worldwide. Methods were developed for analysis of DEG and its potential metabolites; ethylene glycol, glycolic acid, oxalic acid, diglycolic acid and hydroxyethoxy acetic acid in human urine, serum and cereb...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical toxicology 2014-05, Vol.38 (4), p.184-193
Main Authors: Perala, Adam W., Filary, Mark J., Bartels, Michael J., McMartin, Kenneth E.
Format: Article
Language:English
Subjects:
Animals
Calibration
Ethylene Glycols - metabolism
Ethylene Glycols - pharmacokinetics
Ethylene Glycols - poisoning
Female
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
Humans
Kidney - drug effects
Kidney - metabolism
Liver - drug effects
Liver - metabolism
Male
Poisoning - blood
Poisoning - cerebrospinal fluid
Poisoning - urine
Rats, Wistar
Reference Standards
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The misuse of the commonly used chemical diethylene glycol (DEG) has lead to many poisonings worldwide. Methods were developed for analysis of DEG and its potential metabolites; ethylene glycol, glycolic acid, oxalic acid, diglycolic acid and hydroxyethoxy acetic acid in human urine, serum and cerebrospinal fluid samples, collected following a DEG-associated poisoning in the Republic of Panama during 2006. In addition, methods were developed for rat blood, urine, kidney and liver tissue to support toxicokinetic analysis during the conduct of DEG acute toxicity studies in the rat. Sample analysis was conducted using two techniques; ion chromatography with suppressed conductivity and negative ion electrospray ionization with MS detection or with gas chromatography using electron impact ionization or methane negative chemical ionization with MS detection. Stable-isotope-labeled analogs of each analyte were employed as quantitative internal standards in the assays.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/bku018