A novel angiotensin-I converting enzyme inhibitory peptide derived from the glutelin of vinegar soaked black soybean and its antihypertensive effect in spontaneously hypertensive rats

• Published in: Journal of biochemistry (Tokyo) 2019-09, Vol.166 (3), p.223-230
• Main Authors: Zhang, Yueyuan, Zhang, Yanling, Chen, Peiyao, Shu, Fengjue, Li, Kai, Qiao, Liansheng, Chen, Zujun, Wang, Lingzhi
• Format: Article
• Language: English
• Subjects: Acetic Acid - chemistry; Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Antihypertensive Agents - chemistry; Antihypertensive Agents - isolation & purification; Antihypertensive Agents - pharmacology; Dose-Response Relationship, Drug; Glutens - chemistry; Glutens - isolation & purification; Glycine max - chemistry; Hypertension - drug therapy; Hypertension - metabolism; Male; Molecular Docking Simulation; Oligopeptides - chemistry; Oligopeptides - isolation & purification; Oligopeptides - pharmacology; Peptidyl-Dipeptidase A - metabolism; Rats; Rats, Inbred SHR; Structure-Activity Relationship
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/jb/mvz029

Vinegar soaked black soybean is a traditional Chinese food widely used for the treatment of hypertension. While its pharmacodynamic substance was not fully unveiled. It contained abundant glutelin, thus the purpose of this study was to obtain potent antihypertensive peptides from vinegar soaked black soybean. Black soybean was soaked with vinegar and then glutelin was first catalyzed by alcalase. Ultrafiltration, ion exchange chromatography and reversed-phase high performance liquid chromatography were sequentially applied to separate and purify the angiotensin-I converting enzyme (ACE) inhibitory peptides from glutelin hydrolysates. As a result, the fraction L1-4 with the highest ACE inhibitory activity (83.41%) at the final concentration of 0.01 mg/ml was obtained and five peptides were then identified. These peptides were further optimized by virtual screening combining with in silico proteolysis. Finally, a novel tetrapeptide Phe-Gly-Ser-Phe (FGSF) was obtained. FGSF exhibited high in vitro ACE inhibitory activity (IC50 = 117.11 μM) and in vivo hypotensive effect which maximally reduced systolic blood pressure of 21.95 mmHg at 20 mg/kg body weight in spontaneously hypertensive rats. Our study demonstrated that FGSF derived from vinegar soaked black soybean might be used as a promising ingredient for pharmaceuticals against hypertension and its related diseases.