536 Glucocorticoid Prevents Apoptosis of Heat-injured Fibroblast Cells in Vitro Experiment Model

• Published in: Journal of burn care & research 2018-04, Vol.39 (suppl_1), p.S241-S242
• Main Authors: Matsuura, Y, Kawai, K, Kawabata, S, Noda, K, Suzuki, S
• Format: Article
• Language: English
• ISSN: 1559-047X; 1559-0488
• DOI: 10.1093/jbcr/iry006.459

Abstract Introduction Superficial partial-thickness burn wounds tend to progress to deep partial-thickness burn wounds unless treated. Also, the partial thickness burn has both necrosis and apoptosis of fibroblast cells. Suppression of the cell death may prevent the burn progression. The purpose of this study is to investigate the heated fibroblast’s area, so the change of fibroblasts to necrosis and apoptosis timely in the experimental burn model. Also, we examined the effect of glucocorticoid whether fibroblasts after heated prevent the cell death or not. Methods We developed in vitro experimental model of heat-injured fibroblasts produced by temperature-controlled metal device. The cells were taken from rat’s skin. We set the three conditions to examine the change to the dead cell, necrosis or apoptosis after heated. The setting ones ware 41°C for 30seconds, 46°C for 30seconds and 55°C for 30seconds. We counted the number of the live cell and apoptosis and necrosis after 24hours burn. And then, we used betamethasone 100ng/ml to examine the effect of glucocorticoid whether the cell death was decreased or not after burn. The numbers of apoptosis and necrosis and the live cells was statically analyzed between control group and betamethasone group. Results The number of necrosis was gradually decreased and the one of necrosis was increased as the temperature rose. The other hand, the more apoptosis was seen at the setting of 46°C than at the setting of 41°C and 55°C. Also, in the case of using betamethasone100ng/ml, the less apoptosis and the more live cells ware observed statically compared with control group. The number necrosis was not changed between control group and betamethasone group. Conclusions We developed in vitro experimental model of heat-injured fibroblasts. The number of apoptosis was most at the setting 46°C among 41°C, 46°C and 55°C. Betamethasone has the possibility to prevent apoptosis from fibroblasts after heated in vitro burn model. Applicability of Research to Practice Agents of anti-apoptosis effect may prevent burn progression. So, dressing burn wounds with glucocorticoid has usefulness in the primary burn therapy.