Cell division heterogeneity in the primary meristems of Pisum sativum root tips after excision

Root tips (1.0 cm) were excised, transferred to culture medium, sampled at intervals, embedded in paraffin and sectioned. The mitotic index in each primary meristem in the 2.0 mm tips was reduced immediately after excision. After 8–12 h mitosis resumed. The position of mitotic figures in the protode...

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Bibliographic Details
Published in:Journal of experimental botany 1982-12, Vol.33 (137), p.1312-1318
Main Authors: Rost, T.L, Rowland, J, Dewees, C
Format: Article
Language:English
Subjects:
Cell division
Cell growth
Guard cells
Meristems
Mitotic index
Peas
Plant tissues
Root cap
Root tips
Statistical median
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Summary:Root tips (1.0 cm) were excised, transferred to culture medium, sampled at intervals, embedded in paraffin and sectioned. The mitotic index in each primary meristem in the 2.0 mm tips was reduced immediately after excision. After 8–12 h mitosis resumed. The position of mitotic figures in the protoderm, ground meristem, procambium, and root cap was determined. The greatest number of mitotic figures occurred in the procambium, and upon excision the smallest percent reduction also occurred in this region. The ground meristem showed the greatest reduction in mitotic figures. After excision, the relative contribution to the total number of mitotic figures increased in the procambium tissue and decreased in the ground meristem. Cell division in all primary meristems of the root tip is reduced after tip excision, but the ground meristem (cortical region) is the primary target tissue.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/33.6.1312