Characteristics of transport of L-leucine and glycine in pea protoplasts

• Published in: Journal of experimental botany 1984-07, Vol.35 (156), p.1022-1031
• Main Authors: Dureja, I, Guha Mukherjee, S, Prasad, R
• Format: Article
• Language: English
• Subjects: Absorption. Translocation of ions and substances. Permeability; Active transport; Agronomy. Soil science and plant productions; Amino acids; Arsenates; Azides; Biological and medical sciences; Cultured cells; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Glycine transport; Kinetics; L-Leucine; Mediated transport; Nutrition. Photosynthesis. Respiration. Metabolism; Pea protoplast; Peas; plant biochemistry; plant physiology; Plant physiology and development; Plants; Protoplasts; Sodium; Water and solutes. Absorption, translocation and permeability
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/35.7.1022

The uptake of L-leucine and glycine into pea protoplasts was studied under various conditions. The uptake of both L-leucine and glycine was pH dependent with the optimal pH being 4.0 and 5.0 for L-leucine and glycine, respectively. A kinetic study of L-leucine uptake showed that uptake is multiphasic; Km values of different phases were 1.1 mol m−3, 33.3 mol m−3 and 100 mol m3. A similar analysis for glycine at a concentration range of 0.1–10 mol m−3 also showed a multiphasic transport system for it. The uptake of L-leucine at lower concentrations(between 0.1–2.0 mol m−3) was energy dependent, since arsenate, azide, dinitrophenol and iodoacetate inhibited the uptake. However, the uptake of L-leucine was not inhibited by ouabain at any concentration of L-leucine employed. The uptake of glycine was not inhibited by any of these inhibitors suggesting that glycine uptake was not mediated by an active process.