Protein dysfunction and inhibition of amino acid incorporation in root segments from wheat and mung bean seedlings caused by some amino acid analogues

The effects of para-fluorophenylalanine (p-FPA) and azetidine-2-carboxylic acid (AZ) on acid phosphatase and peroxidase activity, and on L-leucine incorporation, in root segments of Triticum aestivum L. cv. Fenman and Vigna radial a (L.) Wilczek, were studied. Incubation with 50 mmol−3 AZ significan...

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Bibliographic Details
Published in:Journal of experimental botany 1985-06, Vol.36 (167), p.911-918
Main Authors: Macklon, A.E.S, Vaughan, D, Sim, A, McFarlane, I.D, Ord, B.G
Format: Article
Language:English
Subjects:
Absorption. Translocation of ions and substances. Permeability
Agronomy. Soil science and plant productions
Amino acids
analogs
Azetidine-2-carboxylic acid
Barley
Biological and medical sciences
Economic plant physiology
Enzyme activity
Enzymes
Fluorophenylalanin
Fundamental and applied biological sciences. Psychology
inhibition
Isomers
Metabolism
Metabolism. Physicochemical requirements
Nutrient solutions
Nutrition. Photosynthesis. Respiration. Metabolism
Phosphatases
Plant physiology
Plant physiology and development
Plant roots
protein metabolism
roots
Seedlings
Triticum aestivum
Vigna radiata
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Summary:The effects of para-fluorophenylalanine (p-FPA) and azetidine-2-carboxylic acid (AZ) on acid phosphatase and peroxidase activity, and on L-leucine incorporation, in root segments of Triticum aestivum L. cv. Fenman and Vigna radial a (L.) Wilczek, were studied. Incubation with 50 mmol−3 AZ significantly reduced phosphatase and peroxidase activities in wheat roots, but with 20 mol m−3 p-FPA, only the peroxidase activity was reduced. In mung bean roots, phosphatase activity was inhibited by both AZ and p-FPA. Effects of the ortho- and meta- isomers of FPA on wheat root phosphatase and peroxidase, and on mung bean phosphatase, were also examined. Leucine uptake and incorporation were not inhibited by 5 h pre-incubation with either p-FPA or AZ, but were inhibited after 24 h of pre-incubation. The results support the view that, in the shorter term, the analogues inhibit enzyme activity by becoming incorporated to produce non-functional protein and, in the longer term, metabolism is further affected by inhibition of protein synthesis.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/36.6.911