Mechanism of L-lysine transport by pea protoplasts

Dureja, I., Guha-Mukherjee, S. and Prasad, R. 1986. Mechanism of L-lysine transport by pea protoplasts.—J. exp. Bot. 37: 549–555. L-Lysine uptake was studied in pea protoplasts to characterize the transport process. The uptake was pH dependent with optimum at pH 5·8. A kinetic analysis of uptake sho...

Full description

Saved in:
Bibliographic Details
Published in:Journal of experimental botany 1986-04, Vol.37 (177), p.549-555
Main Authors: Dureja, I, Guha-Mukherjee, S, Prasad, R
Format: Article
Language:English
Subjects:
Acidic amino acids
active transport
Amino acids
Basic amino acids
Cultured cells
Kinetics
L-lysine transport
lysine
Mesophyll cells
Pea protoplasts
Peas
Pisum sativum
Plant cells
Plant physiology
Protoplasts
specific system
transport
uptake
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dureja, I., Guha-Mukherjee, S. and Prasad, R. 1986. Mechanism of L-lysine transport by pea protoplasts.—J. exp. Bot. 37: 549–555. L-Lysine uptake was studied in pea protoplasts to characterize the transport process. The uptake was pH dependent with optimum at pH 5·8. A kinetic analysis of uptake showed that L-lyslne uptake was biphasic. The respiratory inhibitors, sodium arsenate, azide, iodoacetate and 2, 4, dinitrophenol, inhibited the uptake of L-lysine at a final concentration of 0·1 mol m−3 suggesting it to be mediated in part by an active process. Competitive inhibition of L-lysine uptake by only L-arglnine and of L-leucine and glycine uptake by several amino acids indicated that L-lysine uptake occurs via a specific system whereas the uptake of L-leucine and glycine was mediated through a relatively non-specific permease.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/37.4.549