Induction of low molecular weight cadmium-binding compound in soybean roots

Blakeley, S. D., Robaglia, C, Brzezinski, R. and Thirion, J-P. 1986. Induction oflow molecular weight cadmium-binding compound in soybean roots.—J. exp. Bot. 37: 956–964. A Cd-binding compound has been identified in roots of Cd-treated soybean plants (Glycine max L.). Induction of synthesis of this...

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Bibliographic Details
Published in:Journal of experimental botany 1986-07, Vol.37 (180), p.956-964
Main Authors: Blakeley, S.D, Robaglia, C, Brzezinski, R, Thirion, J.P
Format: Article
Language:English
Subjects:
Agrochemicals products
binding
Biological and medical sciences
Cabbages
cadmium
Chemical constitution
Electrophoresis
Fundamental and applied biological sciences. Psychology
Gels
Glycine max
induction
Low molecular weights
Molecular weight
Other agrochemicals used in plant protection (herbicides, fungicides, etc.)
Phytopathology. Animal pests. Plant and forest protection
Plant physiology and development
Plant roots
Plants
Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors
Pollution effects. Side effects of agrochemicals
Proteins
Radioactive decay
root
roots
Soybean
Soybeans
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Summary:Blakeley, S. D., Robaglia, C, Brzezinski, R. and Thirion, J-P. 1986. Induction oflow molecular weight cadmium-binding compound in soybean roots.—J. exp. Bot. 37: 956–964. A Cd-binding compound has been identified in roots of Cd-treated soybean plants (Glycine max L.). Induction of synthesis of this compound after treatment with CdCl2 was monitored by labelling with either 109Cd in vitro or with 35S-cysteine or 35S-methionine in vivo. The apparent molecular weight as determined by gel filtration was about 14000 daltons. However, after carboxymethylation and electrophoresis under denaturing conditions its molecular weight was less than 3400 daltons. Simultaneous labelling with 109 and 35S-cysteine demonstrated that the compound contains cysteine residues. Gel electrophoresis of 35S-cysteine or 35S-methionine labelled protein samples showed the compound to be rich in cysteine but not in methionine, and that it was induced about 60-fold by 1·0 mol m−3 CdCl2 after 7 d.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/37.7.956