effects of fusicoccin on anion fluxes in isolated guard cells of Commelina communis L

• Published in: Journal of experimental botany 1987-05, Vol.38 (190), p.863-876
• Main Author: Clint, G.M
• Format: Article
• Language: English
• Subjects: Anions; Biological and medical sciences; Bromides; Cell membranes; Commelina communis; Cytoplasm; Fundamental and applied biological sciences. Psychology; Fusicoccin; Guard cells; ion fluxes; Ions; Plant physiology and development; Standard error; Tonoplast; Vacuoles; Water and solutes. Absorption, translocation and permeability
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/38.5.863

Clint, G. M. 1987. The effects of fusicoccin on anion fluxes in isolated guard cells of Commelina communis L.—J. exp. BoL 38: 863–876. The effects of 3×10−2 mol m−3 fusicoccin (FC) on bromide fluxes and contents in isolated guard cells of Commelina communis L. have been studied using K82Br at pH 3·9 and pH 6·7. At pH 3·9 FC caused a reduction in both the influx and the efflux of 82Br, whereas at pH 6·7 FC had no effect on the influx but caused a transient increase in the efflux of 82Br. There was no obvious change in bromide content with FC treatment at either pH. The behaviour of the anion fluxes in response to FC suggests that FC does not act solely via a hyperpolarization at the plasmalemma. A redistribution of bromide between the intracellular compartments suggests that anion flux from the cytoplasm to the vacuole may be stimulated by FC at pH 3·9. The failure of guard cells to increase their anion content on treatment with FC despite an increase in stomatal aperture and in cation content suggests that in FC-induced stomatal opening excess cation is balanced by organic acid synthesis within the guard cell.