Cold acclimation and low temperature resistance in cotton: Gossypium hirsutum phospholipase Dα isoforms are differentially regulated by temperature and light

Phospholipase Dα (PLDα) was isolated from cultivated cotton (Gossypium hirsutum) and characterized. Two PLDα genes were identified in the allotetraploid genome of G. hirsutum, derived from its diploid progenitors, G. raimondii and G. arboreum. The genes contained three exons and two introns. The tra...

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Bibliographic Details
Published in:Journal of experimental botany 2010-06, Vol.61 (11), p.2991-3002
Main Authors: Kargiotidou, Anastasia, Kappas, Ilias, Tsaftaris, Athanasios, Galanopoulou, Dia, Farmaki, Theodora
Format: Article
Language:English
Subjects:
Acclimatization
Alternative splicing
Biological and medical sciences
cold acclimation
cold stress
Complementary DNA
cotton
Cotyledons
Freezing
Fundamental and applied biological sciences. Psychology
Genomes
Genomics
lipid modification
Lipids
Low temperature
phospholipase Dα
Plants
Protein isoforms
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Summary:Phospholipase Dα (PLDα) was isolated from cultivated cotton (Gossypium hirsutum) and characterized. Two PLDα genes were identified in the allotetraploid genome of G. hirsutum, derived from its diploid progenitors, G. raimondii and G. arboreum. The genes contained three exons and two introns. The translated products shared a 98.6% homology and were designated as GrPLDα and GaPLDα. Their ORFs encoded a polypeptide of 807 amino acids with a predicted molecular mass of 91.6 kDa sharing an 81-82% homology with PLDα1 and PLDα2 from A. thaliana. A possible alternative splicing event was detected at the 5' untranslated region which, however, did not result in alternative ORFs. Cold stress (10 °C or less) resulted in gene induction which was suppressed below control levels (25 °C or 22 °C growth temperature) when plants were acclimated at 17 °C before applying the cold treatment. Differences in the expression levels of the isoforms were recorded under cold acclimation, and cold stress temperatures. Expression was light regulated under growth, acclimation, and cold stress temperatures. Characterization of the products of lipid hydrolysis by the endogenous PLDα indicated alterations in lipid species and a variation in levels of the signalling molecule phosphatidic acid (PA) following acclimation or cold stress.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erq124