Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor

• Published in: Molecular human reproduction 2002-10, Vol.8 (10), p.912-918
• Main Authors: Goud, P.T., Goud, A.P., Leybaert, L., Oostveldt, P.Van, Mikoshiba, K., Diamond, M.P., Dhont, M.
• Format: Article
• Language: English
• Subjects: 5-trisphosphate receptors; Biological and medical sciences; Ca2+ transients; caged IP3; fertilization; flash photolysis; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; inositol 1; Mammalian female genital system; Vertebrates: reproduction
• ISSN: 1360-9947; 1460-2407; 1460-2407
• DOI: 10.1093/molehr/8.10.912

Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP3R) are expressed in human oocytes and may be involved in operating the Ca2+ release triggered by the fertilizing sperm. This study examines the contribution of type I InsP3R in operating Ca2+ release in human oocytes secondary to InsP3 itself, using a specific function-blocking antibody in conjunction with photolytic release of microinjected InsP3. Intracellular Ca2+ responses were assessed in oocytes microinjected with only caged InsP3 in experiment set A, while in experiment sets B and C, sibling oocytes were injected with caged InsP3 and the blocking antibody or a corresponding volume of medium, prior to flash photolysis. In experiment set C, certain fertilization-related phenomena (cortical granule exocytosis and chromatin configurations) were assessed using optical sections and three-dimensional image reconstructions obtained from a confocal laser scanning microscope. In experiment set A, photolytic release of InsP3 triggered a Ca2+ response (increase from ∼100 to 220 nmol/l followed by an exponential recovery, n = 8) and a wave in the oocytes that spread from the stimulation point to the opposite pole. In set B, photolytic InsP3 release generated Ca2+ responses in control oocytes (n = 9), but not in the antibody-injected oocytes (n = 7). In set C, cortical granule exocytosis and anaphase chromosome configurations were noted in the control oocytes after flash photolysis (n = 6). These changes were completely absent in antibody injected oocytes as their cortical granules were intact and the chromosomes were in metaphase. These oocytes had also lacked Ca2+ responses as in set B (n = 5). This study demonstrates the functional presence of type I InsP3R-operated Ca2+ channels in human oocytes and further suggests an active role of InsP3 in triggering the Ca2+ rise and secondary activation phenomena at fertilization.