Laser crosslinking of E.coli RNA polymerase and T7 DNA

The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration...

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Bibliographic Details
Published in:Nucleic acids research 1982-04, Vol.10 (7), p.2399-2414
Main Authors: Harrison, Catherine A., Turner, Douglas H., Hinkle, David C.
Format: Article
Language:English
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Summary:The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration, and is a linear function of laser intensity. The protein subunits crosslinked to DNA are β, β' and δ.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/10.7.2399