Nucleotide sequence of the transcriptions initiation region of Dictyostelium discoideum rRNA gene and comparison of the initiation regions of three lower eukaryotes’genes

The 5’ end of the rRNA precursor of D. discoideumwas mapped on a cloned rDNA by S1 nuclease protection mapping, and the sequence of about 1240 nucleotides surrounding the transcriptional initiation site of the rRNA gene has been determined. Repeated sequences consisting of 16 nucleotides appeared in...

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Bibliographic Details
Published in:Nucleic acids research 1983-03, Vol.11 (6), p.1725-1734
Main Authors: Hoshikawa, Yoshiko, Iida, Yoichi, lwabuchi, Masaki
Format: Article
Language:English
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Summary:The 5’ end of the rRNA precursor of D. discoideumwas mapped on a cloned rDNA by S1 nuclease protection mapping, and the sequence of about 1240 nucleotides surrounding the transcriptional initiation site of the rRNA gene has been determined. Repeated sequences consisting of 16 nucleotides appeared in the region upstream from the initiation point. Comparison of the nucleotide sequences around the initiation site of rRNA genes in three lower eukaryotes, D. discoideum, Saccharomyces cerevisiaeand Terahymena pyriformis,indicated that there was little similarity in the nontranscribed spacer regions, but in the transcribed spacer regions near the initiation point, very similar sequences consisting of 9 nucleotides were found.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/11.6.1725